Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SGTB/SGT2 antibody [EPR17183] (ab202419)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17183] to SGTB/SGT2
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SGTB/SGT2 antibody [EPR17183]
    See all SGTB/SGT2 primary antibodies

  • Description

    Rabbit monoclonal [EPR17183] to SGTB/SGT2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    IP
    Mouse
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, PC-12 and NIH/3T3 cell lysates; Human fetal brain, mouse brain and rat brain lysates. IHC-P: Human, mouse and rat cerebral cortex tissues. ICC/IF: SH-SY5Y and U-87 MG cells. IP: Mouse brain whole cell lysate.

  • General notes

     

     

     This product was previously labelled as SGTB

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17183

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-SGTB/SGT2 antibody [EPR17183] (ab202419)
    Western blot - Anti-SGTB/SGT2 antibody [EPR17183] (ab202419)
    Anti-SGTB/SGT2 antibody [EPR17183] (ab202419) at 1/2000 dilution + HepG2 (Human liver hepatocellular carcinoma) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature PMID: 12878599.

  • Western blot - Anti-SGTB/SGT2 antibody [EPR17183] (ab202419)
    Western blot - Anti-SGTB/SGT2 antibody [EPR17183] (ab202419)
    Anti-SGTB/SGT2 antibody [EPR17183] (ab202419) at 1/2000 dilution + Human fetal brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa
    Observed band size: 37 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature PMID: 12878599.

  • Western blot - Anti-SGTB/SGT2 antibody [EPR17183] (ab202419)
    Western blot - Anti-SGTB/SGT2 antibody [EPR17183] (ab202419)
    All lanes : Anti-SGTB/SGT2 antibody [EPR17183] (ab202419) at 1/2000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) cell lysate
    Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa
    Observed band size: 37 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature PMID: 12878599.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGTB/SGT2 antibody [EPR17183] (ab202419)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGTB/SGT2 antibody [EPR17183] (ab202419)

    Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling SGTB/SGT2 with ab202419 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGTB/SGT2 antibody [EPR17183] (ab202419)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGTB/SGT2 antibody [EPR17183] (ab202419)

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling SGTB/SGT2 with ab202419 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on mouse cerebral cortex tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGTB/SGT2 antibody [EPR17183] (ab202419)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGTB/SGT2 antibody [EPR17183] (ab202419)

    Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling SGTB/SGT2 with ab202419 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on rat cerebral cortex tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SGTB/SGT2 antibody [EPR17183] (ab202419)
    Immunocytochemistry/ Immunofluorescence - Anti-SGTB/SGT2 antibody [EPR17183] (ab202419)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling SGTB/SGT2 with ab202419 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear and weakly cytoplasmic staining on SH-SY5Y cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab202419 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-SGTB/SGT2 antibody [EPR17183] (ab202419)
    Immunocytochemistry/ Immunofluorescence - Anti-SGTB/SGT2 antibody [EPR17183] (ab202419)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling SGTB/SGT2 with ab202419 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear and cytoplasmic staining on U-87 MG cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab202419 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Immunoprecipitation - Anti-SGTB/SGT2 antibody [EPR17183] (ab202419)
    Immunoprecipitation - Anti-SGTB/SGT2 antibody [EPR17183] (ab202419)

    SGTB/SGT2 was immunoprecipitated from 1mg of Mouse brain whole cell lysate with ab202419 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab202419 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Mouse brain whole cell lysate 10 µg (Input). Lane 2: ab202419 IP in Mouse brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab202419 in Mouse brain whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Anti-SGTB/SGT2 antibody [EPR17183] (ab202419)
    Anti-SGTB/SGT2 antibody [EPR17183] (ab202419)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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