Anti-CCT2 antibody [EPR4083] (ab109184)
![Anti-CCT2 antibody [EPR4083] (ab109184)](https://www.abcam.com/ps/products/109/ab109184/Images/ab109184-121767-anti-tcp1-beta-antibody-epr4083-western-blot.jpg "Anti-CCT2 antibody [EPR4083] (ab109184)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4083] to CCT2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CCT2 antibody [EPR4083]
See all CCT2 primary antibodies -
Description
Rabbit monoclonal [EPR4083] to CCT2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, MCF-7 and A431 cell lysates; Human kidney tissue
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General notes
This product was previously labelled as TCP1 beta
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4083 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CCT2 antibody [EPR4083] (ab109184)All lanes : Anti-CCT2 antibody [EPR4083] (ab109184) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 57 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-CCT2 antibody [EPR4083] (ab109184)](https://www.abcam.com/ps/products/109/ab109184/Images/ab109184-261325-anti-tcp1-beta-antibody-epr4083-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CCT2 antibody [EPR4083] (ab109184)Immunofluorescence staining of HeLa cells with purified ab109184 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 100% methanol. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4083] (ab109184)](https://www.abcam.com/ps/products/109/ab109184/Images/ab109184-121766-anti-tcp1-beta-antibody-epr4083-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4083] (ab109184)ab109184, at 1/100 dilution, staining CCT2 in Human kidney tissue by Immunohistochemistry
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-CCT2 antibody [EPR4083] (ab109184)](https://www.abcam.com/ps/products/109/ab109184/Images/ab109184-4-benefits-of-recombinant-antibodies.png)
Anti-CCT2 antibody [EPR4083] (ab109184)
