Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CCT2 antibody [EPR4083] (ab109184)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4083] to CCT2
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CCT2 antibody [EPR4083]
    See all CCT2 primary antibodies

  • Description

    Rabbit monoclonal [EPR4083] to CCT2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, MCF-7 and A431 cell lysates; Human kidney tissue

  • General notes

     This product was previously labelled as TCP1 beta

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR4083

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CCT2 antibody [EPR4083] (ab109184)
    Western blot - Anti-CCT2 antibody [EPR4083] (ab109184)
    All lanes : Anti-CCT2 antibody [EPR4083] (ab109184) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 57 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CCT2 antibody [EPR4083] (ab109184)
    Immunocytochemistry/ Immunofluorescence - Anti-CCT2 antibody [EPR4083] (ab109184)

    Immunofluorescence staining of HeLa cells with purified ab109184 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 100% methanol. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4083] (ab109184)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4083] (ab109184)

    ab109184, at 1/100 dilution, staining CCT2 in Human kidney tissue by Immunohistochemistry

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-CCT2 antibody [EPR4083] (ab109184)
    Anti-CCT2 antibody [EPR4083] (ab109184)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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