Anti-CCT2 antibody (ab225879)
Key features and details
- Rabbit polyclonal to CCT2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CCT2 antibody
See all CCT2 primary antibodies -
Description
Rabbit polyclonal to CCT2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human CCT2 aa 484-534. The exact sequence is proprietary. (NP_006422.1).
Database link: P78371 -
Positive control
- WB: HEK-293T, Jurkat, HeLa and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
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General notes
This product was previously labelled as TCP1 beta
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab225879 was affinity purified using an epitope specific to CCT2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CCT2 antibody (ab225879)All lanes : Anti-CCT2 antibody (ab225879) at 0.04 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 57 kDa
Exposure time: 10 seconds
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Immunoprecipitation - Anti-CCT2 antibody (ab225879)CCT2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225879 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225879 at 0.4 µg/ml.
Lane 1: ab225879 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
