Anti-eIF4E (phospho S209) antibody (ab131513)
Key features and details
- Rabbit polyclonal to eIF4E (phospho S209)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-eIF4E (phospho S209) antibody
See all eIF4E primary antibodies -
Description
Rabbit polyclonal to eIF4E (phospho S209) -
Host species
Rabbit -
Specificity
ab131513 detects endogenous levels of eIF4E only when phosphorylated at Serine 209. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide conjugated to KLH, corresponding to a region around the phosphorylation site of Serine 209 (S-G-Sp-T-T) of Human eIF4E (NP_001124150.1).
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Positive control
- Methanol fixed MCF cells; HT29 cell extracts treated with Anisomycin; Human breast carcinoma tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab131513 was purified using epitope specific phosphopeptide. Non phospho specific antibodies were removed by chromatography using non phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-eIF4E (phospho S209) antibody (ab131513)All lanes : Anti-eIF4E (phospho S209) antibody (ab131513) at 1/500 dilution
Lane 1 : HT29 cell extracts, untreated
Lane 2 : HT29 cell extracts Anisomycin treated
Predicted band size: 25 kDa
For western blotting, membrane should be incubated with diluted antibody in 5% nonfat milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking overnight. -
Immunocytochemistry/ Immunofluorescence - Anti-eIF4E (phospho S209) antibody (ab131513)Immunofluorescence analysis of eIF4E (phospho S209) in methanol fixed MCF cells stained with ab131513 at a dilution of 1/100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4E (phospho S209) antibody (ab131513)Immunohistochemical analysis of eIF4E (phospho S209) in paraffin embedded Human breast carcinoma tissue stained with ab131513 at a 1/50 dilution (left image), or preincubated with immunizing peptide (right image).
