Anti-FNDC5 antibody (ab131390)
Key features and details
- Rabbit polyclonal to FNDC5
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FNDC5 antibody
See all FNDC5 primary antibodies -
Description
Rabbit polyclonal to FNDC5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Horse, Chicken, Cow, Dog, Pig, Zebrafish, Macaque monkey, Orangutan
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Immunogen
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Positive control
- This antibody gave a positive signal in the following whole cell lysates: K562; C2C12.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 23 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 23 kDa).Target
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Sequence similarities
Contains 1 fibronectin type-III domain. -
Cellular localization
Peroxisome membrane. Imported in peroxisomes through the PEX5 receptor pathway. - Information by UniProt
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Database links
- Entrez Gene: 252995 Human
- Entrez Gene: 384061 Mouse
- Entrez Gene: 260327 Rat
- Entrez Gene: 555635 Zebrafish
- Omim: 611906 Human
- SwissProt: Q8NAU1 Human
- SwissProt: Q8K4Z2 Mouse
- SwissProt: Q8K3V5 Rat
see all -
Alternative names
- Fibronectin type III domain containing 5 antibody
- Fibronectin type III domain-containing protein 5 antibody
- Fibronectin type III repeat containing protein 2 antibody
see all
Images
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Western blot - Anti-FNDC5 antibody (ab131390)All lanes : Anti-FNDC5 antibody (ab131390) at 1 µg/ml
Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 2 : C2C12 (Mouse myoblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab131390 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Western blot - Anti-FNDC5 antibody (ab131390)All lanes : Anti-FNDC5 antibody (ab131390) at 1 µg/ml
Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 2 : C2C12 (Mouse myoblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab131390 has been referenced in 15 publications.
- Li HS et al. Myostain is involved in ginsenoside Rb1-mediated anti-obesity. Pharm Biol 60:1106-1115 (2022). PubMed: 35639355
- Wu J et al. Pentamethylquercetin Regulates Lipid Metabolism by Modulating Skeletal Muscle-Adipose Tissue Crosstalk in Obese Mice. Pharmaceutics 14:N/A (2022). PubMed: 35745732
- Tu T et al. Irisin Contributes to Neuroprotection by Promoting Mitochondrial Biogenesis After Experimental Subarachnoid Hemorrhage. Front Aging Neurosci 13:640215 (2021). PubMed: 33613273
- Abbate JM et al. Anti-Atherogenic Effect of 10% Supplementation of Anchovy (Engraulis encrasicolus) Waste Protein Hydrolysates in ApoE-Deficient Mice. Nutrients 13:N/A (2021). PubMed: 34206655
- de Oliveira M et al. The roles of triiodothyronine and irisin in improving the lipid profile and directing the browning of human adipose subcutaneous cells. Mol Cell Endocrinol 506:110744 (2020). PubMed: 32027943
Images
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Western blot - Anti-FNDC5 antibody (ab131390)All lanes : Anti-FNDC5 antibody (ab131390) at 1 µg/ml
Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 2 : C2C12 (Mouse myoblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab131390 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Western blot - Anti-FNDC5 antibody (ab131390)All lanes : Anti-FNDC5 antibody (ab131390) at 1 µg/ml
Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 2 : C2C12 (Mouse myoblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
