Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FNDC5 antibody (ab131390)

Key features and details

  • Rabbit polyclonal to FNDC5
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-FNDC5 antibody
    See all FNDC5 primary antibodies

  • Description

    Rabbit polyclonal to FNDC5

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Cow, Dog, Pig, Zebrafish, Macaque monkey, Orangutan

  • Immunogen

    Synthetic peptide corresponding to Mouse FNDC5 aa 50-150 conjugated to keyhole limpet haemocyanin.
    Database link: Q8K4Z2
    (Peptide available as ab153677)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: K562; C2C12.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab131390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 23 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 23 kDa).

Target

Images

  • Western blot - Anti-FNDC5 antibody (ab131390)
    Western blot - Anti-FNDC5 antibody (ab131390)
    All lanes : Anti-FNDC5 antibody (ab131390) at 1 µg/ml

    Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 2 : C2C12 (Mouse myoblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 22 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab131390 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Western blot - Anti-FNDC5 antibody (ab131390)
    Western blot - Anti-FNDC5 antibody (ab131390)
    All lanes : Anti-FNDC5 antibody (ab131390) at 1 µg/ml

    Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 2 : C2C12 (Mouse myoblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 22 kDa why is the actual band size different from the predicted?
    Additional bands at: 45 kDa, 50 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes

References (15)

Publishing research using ab131390? Please let us know so that we can cite the reference in this datasheet.

ab131390 has been referenced in 15 publications.

  • Li HS  et al. Myostain is involved in ginsenoside Rb1-mediated anti-obesity. Pharm Biol 60:1106-1115 (2022). PubMed: 35639355
  • Wu J  et al. Pentamethylquercetin Regulates Lipid Metabolism by Modulating Skeletal Muscle-Adipose Tissue Crosstalk in Obese Mice. Pharmaceutics 14:N/A (2022). PubMed: 35745732
  • Tu T  et al. Irisin Contributes to Neuroprotection by Promoting Mitochondrial Biogenesis After Experimental Subarachnoid Hemorrhage. Front Aging Neurosci 13:640215 (2021). PubMed: 33613273
  • Abbate JM  et al. Anti-Atherogenic Effect of 10% Supplementation of Anchovy (Engraulis encrasicolus) Waste Protein Hydrolysates in ApoE-Deficient Mice. Nutrients 13:N/A (2021). PubMed: 34206655
  • de Oliveira M  et al. The roles of triiodothyronine and irisin in improving the lipid profile and directing the browning of human adipose subcutaneous cells. Mol Cell Endocrinol 506:110744 (2020). PubMed: 32027943
View all Publications for this product

Images

  • Western blot - Anti-FNDC5 antibody (ab131390)
    Western blot - Anti-FNDC5 antibody (ab131390)
    All lanes : Anti-FNDC5 antibody (ab131390) at 1 µg/ml

    Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 2 : C2C12 (Mouse myoblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 22 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab131390 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Western blot - Anti-FNDC5 antibody (ab131390)
    Western blot - Anti-FNDC5 antibody (ab131390)
    All lanes : Anti-FNDC5 antibody (ab131390) at 1 µg/ml

    Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 2 : C2C12 (Mouse myoblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 22 kDa why is the actual band size different from the predicted?
    Additional bands at: 45 kDa, 50 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-FNDC5 antibody (ab131390)

Clear all

Recently viewed products

Sign up