Antibodies, Proteins, Kits and Reagents for Life Science

345 091 ₸ Product size

100 µl 345 091 ₸ 10 µl 127 315 ₸

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Anti-STUB1/CHIP antibody [EPR4447] (ab134064)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4447] to STUB1/CHIP
Suitable for: WB, IP, Flow Cyt, ICC/IF, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-STUB1/CHIP antibody [EPR4447]
See all STUB1/CHIP primary antibodies
Description

Rabbit monoclonal [EPR4447] to STUB1/CHIP
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human STUB1/CHIP aa 1-100 (N terminal). The exact sequence is proprietary.
Positive control
- HeLa, MCF-7, 293T and ECV-304 cell lysates; Human skeletal muscle tissue.
General notes
Previously labelled as STUB1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4447
Isotype

IgG
Research areas

Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)

All lanes : Anti-STUB1/CHIP antibody [EPR4447] (ab134064) at 1/20000 dilution (purified)

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
Lane 3 : HEK293 (Human epithelial cell line from embryonic kidney) cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 35 kDa
Observed band size: 35 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)

Immunofluorescence staining of SH-SY5Y cells with purified ab134064 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab134064 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
Immunoprecipitation - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)

ab134064 (purified) at 1/120 immunoprecipitating STUB1/CHIP in 10 μg HeLa (Lanes 1 and 2, observed at 35 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labelling STUB1/CHIP with unpurified ab134064 at 1/100 dilution.
Flow Cytometry - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)

Flow Cytometry analysis of SH-SY5Y (Human epithelial cell line from embryonic kidney) cells labeling STUB1/CHIP with purified ab134064 at 1/120 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)

Anti-STUB1/CHIP antibody [EPR4447] (ab134064) at 1/10000 dilution (purified) + Mouse brain at 10 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 35 kDa
Observed band size: 35 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064) This image is courtesy of an anonymous Abreview

All lanes : Anti-STUB1/CHIP antibody [EPR4447] (ab134064) at 1/1000 dilution (unpurified)

Lane 1 : Wild type primary Mouse neurons whole cell lysate
Lane 2 : STUB1 knockout primary Mouse neurons whole cell lysate
Lane 3 : Wild type MEF (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 4 : STUB1 knockout MEF (Mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/20000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 35 kDa

Exposure time: 25 seconds
See Abreview
Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)

Anti-STUB1/CHIP antibody [EPR4447] (ab134064) at 1/50000 dilution (purified) + Rat brain at 10 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 35 kDa
Observed band size: 35 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-STUB1/CHIP antibody [EPR4447] (ab134064)

All lanes : Anti-STUB1/CHIP antibody [EPR4447] (ab134064) at 1/10000 dilution (unpurified)

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : MCF-7 (Human breast adenocarcinoma cell line) cell lysate
Lane 3 : 293T cell lysate
Lane 4 : ECV-304 (Human urinary bladder cancer cell line) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 35 kDa

ECV-304 (derived from T24, discontinued by ATCC)
Anti-STUB1/CHIP antibody [EPR4447] (ab134064)

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