Anti-DNAJB13 antibody (ab185301)
Key features and details
- Rabbit polyclonal to DNAJB13
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DNAJB13 antibody -
Description
Rabbit polyclonal to DNAJB13 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human DNAJB13 aa 150-300.
Database link: P59910 -
Positive control
- IHC-P: Human Fallopian tube, tonsil tissue; WB: Transfected HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJB13 antibody (ab185301)
Immunohistochemistry analysis of human Fallopian tube tissue labeling DNABJ13 (high expression) with ab185301 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-DNAJB13 antibody (ab185301)All lanes : Anti-DNAJB13 antibody (ab185301) at 0.4 µg/ml
Lane 1 : Control (vector only transfected) - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : DNAJB13 over-expression - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate, (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Predicted band size: 36 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJB13 antibody (ab185301)Immunohistochemistry analysis of human tonsil tissue labeling DNABJ13 (low expression) with ab185301 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.