Anti-CLDN16/Claudin-16 antibody (ab106504)
Key features and details
- Rabbit polyclonal to CLDN16/Claudin-16
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CLDN16/Claudin-16 antibody
See all CLDN16/Claudin-16 primary antibodies -
Description
Rabbit polyclonal to CLDN16/Claudin-16 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog, Gorilla
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Immunogen
Synthetic peptide corresponding to Human CLDN16/Claudin-16 aa 250 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab125930) -
Positive control
- This antibody gave a positive signal in HEK293 whole cell lysate as well as both Human Adult and Fetal Kidney tissue lysates. It also gave a positive result in human kidney FFPE tissue sections
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General notes
This product was previously labelled as CLDN16
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab106504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. WB Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). Target
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Function
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. Involved in paracellular magnesium reabsorption. Required for a selective paracellular conductance. May form, alone or in partnership with other constituents, an intercellular pore permitting paracellular passage of magnesium and calcium ions down their electrochemical gradients. Alternatively, it could be a sensor of magnesium concentration that could alter paracellular permeability mediated by other factors. -
Tissue specificity
Kidney-specific, including the thick ascending limb of Henle (TAL). -
Involvement in disease
Defects in CLDN16 are the cause of hypomagnesemia type 3 (HOMG3) [MIM:248250]; also known as familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC). HOMG3 is a progressive renal disease characterized by primary renal magnesium wasting with hypomagnesemia, hypercalciuria and nephrocalcinosis. Recurrent urinary tract infections and kidney stones are often observed. In spite of hypercalciuria, patients do not show hypocalcemia. -
Sequence similarities
Belongs to the claudin family. -
Cellular localization
Cell junction > tight junction. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 10686 Human
- Entrez Gene: 114141 Mouse
- Entrez Gene: 155268 Rat
- Omim: 603959 Human
- SwissProt: Q9Y5I7 Human
- SwissProt: Q925N4 Mouse
- SwissProt: Q91Y55 Rat
- Unigene: 251391 Human
see all -
Alternative names
- Claudin 16 antibody
- Claudin-16 antibody
- CLD16_HUMAN antibody
see all
Images
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Western blot - Anti-CLDN16/Claudin-16 antibody (ab106504)All lanes : Anti-CLDN16/Claudin-16 antibody (ab106504) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Kidney (Human) Tissue Lysate - fetal normal tissue (ab30204)
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Additional bands at: 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLDN16/Claudin-16 antibody (ab106504)IHC image of ab106504 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab106504, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (1)
ab106504 has been referenced in 1 publication.
- Wang X et al. MicroRNA-155-5p is a key regulator of allergic inflammation, modulating the epithelial barrier by targeting PKIa. Cell Death Dis 10:884 (2019). PubMed: 31767859
Images
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Western blot - Anti-CLDN16/Claudin-16 antibody (ab106504)All lanes : Anti-CLDN16/Claudin-16 antibody (ab106504) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Kidney (Human) Tissue Lysate - fetal normal tissue (ab30204)
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Additional bands at: 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLDN16/Claudin-16 antibody (ab106504)IHC image of ab106504 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab106504, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
