Anti-Kallikrein 14 antibody [EPR23570-293] (ab278500)
![Anti-Kallikrein 14 antibody [EPR23570-293] (ab278500)](https://www.abcam.com/ps/products/278/ab278500/Images/ab278500-4-anti-kallikrein-14-antibody-epr23570293-western-blot-lysate.jpg "Anti-Kallikrein 14 antibody [EPR23570-293] (ab278500)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23570-293] to Kallikrein 14
- Suitable for: IHC-P, WB, IHC-Fr
- Reacts with: Mouse
Overview
-
Product name
Anti-Kallikrein 14 antibody [EPR23570-293]
See all Kallikrein 14 primary antibodies -
Description
Rabbit monoclonal [EPR23570-293] to Kallikrein 14 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr MouseIHC-P MouseWB Mouse
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Mouse esophagus tissue lysate; Mouse Kallikrein 14 recombinant protein (aa24-250) 10 ng. IHC-P: Mouse esophagus. IHC-Fr: Mouse esophagus.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23570-293 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Kallikrein 14 antibody [EPR23570-293] (ab278500)All lanes : Anti-Kallikrein 14 antibody [EPR23570-293] (ab278500) at 1/1000 dilution
Lane 1 : Mouse esophagus tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 29 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Blocking buffer and Diluting buffer : 5% NFDM/TBST
The observed MW is consistent with what has been described in the literature (PMID: 17110383).
Negative control: kidney (PMID: 11352573)Exposure time: 26 seconds
-
![Western blot - Anti-Kallikrein 14 antibody [EPR23570-293] (ab278500)](https://www.abcam.com/ps/products/278/ab278500/Images/ab278500-5-anti-kallikrein-14-antibody-epr23570293-western-blot-recombinant.jpg)
Western blot - Anti-Kallikrein 14 antibody [EPR23570-293] (ab278500)Anti-Kallikrein 14 antibody [EPR23570-293] (ab278500) at 1/1000 dilution + Mouse Kallikrein 14 recombinant protein (aa24-250) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 29 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?Blocking buffer and Diluting buffer : 5% NFDM/TBST
Exposure: 10 seconds
Mouse Kallikrein 14 recombinant protein (aa24-250) was expressed from a mammalian - HEK-293 expression system.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 14 antibody [EPR23570-293] (ab278500)](https://www.abcam.com/ps/products/278/ab278500/Images/ab278500-2-anti-kallikrein-14-antibody-epr23570293-immunohistochemistry-mouse-esophagus.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 14 antibody [EPR23570-293] (ab278500)Immunohistochemical analysis of paraffin-embedded Mouse esophagus tissue labelling Thyroid Peroxidase/TPO with ab278500 at 1/5000 (0.585 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. The section was incubated with ab278500 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Frozen sections) - Anti-Kallikrein 14 antibody [EPR23570-293] (ab278500)](https://www.abcam.com/ps/products/278/ab278500/Images/ab278500-1-anti-kallikrein-14-antibody-epr23570293-immunohistochemistry-frozen-mouse-esophagus.jpg)
Immunohistochemistry (Frozen sections) - Anti-Kallikrein 14 antibody [EPR23570-293] (ab278500)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse esophagus tissue labelling kallikrein 14 with ab278500 at 1/100 (5.85 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 14 antibody [EPR23570-293] (ab278500)](https://www.abcam.com/ps/products/278/ab278500/Images/ab278500-3-anti-kallikrein-14-antibody-epr23570293-immunohistochemistry-mouse-kidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 14 antibody [EPR23570-293] (ab278500)Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling Thyroid Peroxidase/TPO with ab278500 at 1/5000 (0.585 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. The section was incubated with ab278500 for 30 mins at room temperature. Negative control: No staining on mouse kidney. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
