Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PER2 antibody [EPR11381(2)] (ab179813)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11381(2)] to PER2
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-PER2 antibody [EPR11381(2)]
    See all PER2 primary antibodies

  • Description

    Rabbit monoclonal [EPR11381(2)] to PER2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PER2 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: O15055

  • Positive control

    • WB: A673, Y79, HeLa and BxPC-3 cell lysates. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-PER2 antibody [EPR11381(2)] (ab179813)
    Western blot - Anti-PER2 antibody [EPR11381(2)] (ab179813)
    All lanes : Anti-PER2 antibody [EPR11381(2)] (ab179813) at 1/5000 dilution (purified)

    Lane 1 : HeLa whole cell lysate
    Lane 2 : A673 whole cell lysate
    Lane 3 : BxPC-3 whole cell lysate
    Lane 4 : Y79 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 137 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.
  • Immunocytochemistry/ Immunofluorescence - Anti-PER2 antibody [EPR11381(2)] (ab179813)
    Immunocytochemistry/ Immunofluorescence - Anti-PER2 antibody [EPR11381(2)] (ab179813)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PER2 with purified ab179813 at a dilution of 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/200) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

  • Flow Cytometry - Anti-PER2 antibody [EPR11381(2)] (ab179813)
    Flow Cytometry - Anti-PER2 antibody [EPR11381(2)] (ab179813)

    Flow Cytometry analysis of HeLa cells labelling PER2 with purified ab179813 at a dilution of 1/200 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Western blot - Anti-PER2 antibody [EPR11381(2)] (ab179813)
    Western blot - Anti-PER2 antibody [EPR11381(2)] (ab179813)
    All lanes : Anti-PER2 antibody [EPR11381(2)] (ab179813) at 1/1000 dilution (unpurified)

    Lane 1 : A673 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : BxPC-3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 137 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PER2 antibody [EPR11381(2)] (ab179813)
    Immunocytochemistry/ Immunofluorescence - Anti-PER2 antibody [EPR11381(2)] (ab179813)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling PER2 with unpurified ab179813 at a dilution of 1/50.

  • Flow Cytometry - Anti-PER2 antibody [EPR11381(2)] (ab179813)
    Flow Cytometry - Anti-PER2 antibody [EPR11381(2)] (ab179813)

    Flow cytometric analysis of permeabilized HeLa cells labeling PER2 with unpurified ab179813 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

  • Anti-PER2 antibody [EPR11381(2)] (ab179813)
    Anti-PER2 antibody [EPR11381(2)] (ab179813)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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