Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HIKESHI antibody [EPR17761] - BSA and Azide free (ab251362)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17761] to HIKESHI - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, ICC
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-HIKESHI antibody [EPR17761] - BSA and Azide free
    See all HIKESHI primary antibodies

  • Description

    Rabbit monoclonal [EPR17761] to HIKESHI - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, ICCmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251362 is the carrier-free version of ab202065.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPR17761

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-HIKESHI antibody [EPR17761] - BSA and Azide free (ab251362)
    Western blot - Anti-HIKESHI antibody [EPR17761] - BSA and Azide free (ab251362)
    Anti-HIKESHI antibody [EPR17761] (ab202065) at 1/1000 dilution + MCF7 (Human breast adenocarcinoma cell line) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 3 minutes


    This data was developed using ab202065, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-HIKESHI antibody [EPR17761] - BSA and Azide free (ab251362)
    Western blot - Anti-HIKESHI antibody [EPR17761] - BSA and Azide free (ab251362)
    Anti-HIKESHI antibody [EPR17761] (ab202065) at 1/1000 dilution + Human fetal heart lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 3 minutes


    This data was developed using ab202065, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-HIKESHI antibody [EPR17761] - BSA and Azide free (ab251362)
    Western blot - Anti-HIKESHI antibody [EPR17761] - BSA and Azide free (ab251362)
    All lanes : Anti-HIKESHI antibody [EPR17761] (ab202065) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse kidney lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 3 minutes


    This data was developed using ab202065, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIKESHI antibody [EPR17761] - BSA and Azide free (ab251362)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIKESHI antibody [EPR17761] - BSA and Azide free (ab251362)
    This data was developed using ab202065, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling HIKESHI with ab202065 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIKESHI antibody [EPR17761] - BSA and Azide free (ab251362)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIKESHI antibody [EPR17761] - BSA and Azide free (ab251362)
    This data was developed using ab202065, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling HIKESHI with ab202065 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on mouse liver tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-HIKESHI antibody [EPR17761] - BSA and Azide free (ab251362)
    Immunocytochemistry - Anti-HIKESHI antibody [EPR17761] - BSA and Azide free (ab251362)
    This data was developed using ab202065, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling HIKESHI with ab202065 at 1/400 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear and cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red). The negative controls are as follows:
    -ve control 1: ab202065 at 1/400 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
  • Immunocytochemistry - Anti-HIKESHI antibody [EPR17761] - BSA and Azide free (ab251362)
    Immunocytochemistry - Anti-HIKESHI antibody [EPR17761] - BSA and Azide free (ab251362)
    This data was developed using ab202065, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling HIKESHI with ab202065 at 1/400 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear and cytoplasmic staining on MCF7 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red). The negative controls are as follows:
    -ve control 1: ab202065 at 1/400 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
  • Immunoprecipitation - Anti-HIKESHI antibody [EPR17761] - BSA and Azide free (ab251362)
    Immunoprecipitation - Anti-HIKESHI antibody [EPR17761] - BSA and Azide free (ab251362)
    This data was developed using ab202065, the same antibody clone in a different buffer formulation.HIKESHI was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab202065 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab202065 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution. Lane 1: MCF7 whole cell lysate 10 µg (Input). Lane 2: ab202065 IP in MCF7 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab202065 in MCF7 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds.
  • Anti-HIKESHI antibody [EPR17761] - BSA and Azide free (ab251362)
    Anti-HIKESHI antibody [EPR17761] - BSA and Azide free (ab251362)

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