Anti-PARK7/DJ1 antibody (ab265596)
Key features and details
- Rabbit polyclonal to PARK7/DJ1
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PARK7/DJ1 antibody
See all PARK7/DJ1 primary antibodies -
Description
Rabbit polyclonal to PARK7/DJ1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human PARK7/DJ1 aa 140-189 (C terminal). The exact sequence is proprietary. NP_009193.2
Database link: Q99497 -
Positive control
- WB: HeLa, HEK-293T, MCF7 whole cell lysate. HeLa S3 cytosolic and nuclear extracts. IHC-P: Human breast carcinoma and mouse teratoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PARK7/DJ1 antibody (ab265596) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 20 µg
Lane 2 : HeLa whole cell lysate at 4 µg
Lane 3 : MCF7 whole cell lysate at 20 µg
Lane 4 : HEK-293T whole cell lysate at 20 µg
Predicted band size: 20 kDa
Exposure time: 5 seconds
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All lanes : Anti-PARK7/DJ1 antibody (ab265596) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 20 kDa
Exposure time: 30 secondsCells prepared using NETN lysis buffer.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling PARK7/DJ1 with ab265596 at 1/1000 dilution. Detection: DAB.
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PARK7/DJ1 was immunoprecipitated from 1mg of HeLa S3 cytosolic (lane 1) and nuclear (lane 2) extract with ab265596 at 0.7 µg/mg lysate.
Detection: vMALDI-LTQ and LC-MS/MS of Coomassie stained bands.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse teratoma tissue labeling PARK7/DJ1 with ab265596 at 1/1000 dilution. Detection: DAB.