Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Aurora B (phospho S227) antibody [EPR20389] (ab210706)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20389] to Aurora B (phospho S227)
Suitable for: WB, Dot blot, ICC/IF, IP
Reacts with: Human

Product name

Anti-Aurora B (phospho S227) antibody [EPR20389]
See all Aurora B primary antibodies
Description

Rabbit monoclonal [EPR20389] to Aurora B (phospho S227)
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa treated with 100 ng/ml nocodazole for 18 hours, whole cell lysate. ICC/IF: HeLa cells. IP: HeLa treated with 100 ng/ml nocodazole for 18 hours, whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20389
Isotype

IgG
Research areas

Western blot - Anti-Aurora B (phospho S227) antibody [EPR20389] (ab210706)

All lanes : Anti-Aurora B (phospho S227) antibody [EPR20389] (ab210706) at 1/1000 dilution

Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa treated with 100 ng/ml nocodazole for 18 hours, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 39 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Aurora B (phospho S227) antibody [EPR20389] (ab210706)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Aurora B (phospho S227) with ab210706 at 1/10000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing midbody staining (arrow) on HeLa cell line.

The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunoprecipitation - Anti-Aurora B (phospho S227) antibody [EPR20389] (ab210706)

Aurora B (phospho S227) was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/ml nocodazole for 18 hours whole cell lysate with ab210706 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab210706 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: HeLa treated with 100 ng/ml nocodazole for 18 hours whole cell lysate 10μg (Input).

Lane 2: ab210706 IP in HeLa treated with 100 ng/ml nocodazole for 18 hours whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab210706 in HeLa treated with 100 ng/ml nocodazole for 18 hours whole cell lysate.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 30 seconds
Dot Blot - Anti-Aurora B (phospho S227) antibody [EPR20389] (ab210706)

Dot blot analysis of Aurora B (phospho S227) labeled with ab210706 at 1/1000 dilution.

Lane 1: Aurora B (phospho S227) peptide.

Lane 2: Aurora B non-phospho peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes
Anti-Aurora B (phospho S227) antibody [EPR20389] (ab210706)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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