Antibodies, Proteins, Kits and Reagents for Life Science

358 492 ₸ Product size

100 µl 358 492 ₸ 10 µl 134 016 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Aurora B antibody [EP1009Y] (ab45145)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1009Y] to Aurora B
Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
Reacts with: Human

Product name

Anti-Aurora B antibody [EP1009Y]
See all Aurora B primary antibodies
Description

Rabbit monoclonal [EP1009Y] to Aurora B
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Aurora B aa 1-100 (N terminal). The exact sequence is proprietary.
Positive control
- IP: HeLa cell lysate
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Dissociation constant (K_D)

K_D = 5.50 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1009Y
Isotype

IgG
Research areas

Flow Cytometry - Anti-Aurora B antibody [EP1009Y] (ab45145)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Aurora B with Purified ab45145 at 1:300 dilution (0.1 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor ® 488 ,ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Left). Unlabeled control - /.
Immunocytochemistry - Anti-Aurora B antibody [EP1009Y] (ab45145)

Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Aurora B with Purified ab45145 at 1:50 dilution (5.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-Aurora B antibody [EP1009Y] (ab45145)

Lanes 1-2 : Anti-Aurora B antibody [EP1009Y] (ab45145) at 1/1000 dilution
Lanes 3-4 : Anti-Aurora B antibody [EP1009Y] (ab45145) at 1/5000 dilution
Lanes 5-6 : Anti-Aurora B antibody [EP1009Y] (ab45145) at 1/10000 dilution
Lanes 7-8 : Anti-Aurora B antibody [EP1009Y] (ab45145) at 1/50000 dilution
Lanes 9-10 : Anti-Aurora B antibody [EP1009Y] (ab45145) at 1/75000 dilution

Lanes 1 & 3 & 5 & 7 & 9 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lanes 2 & 4 & 6 & 8 & 10 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Nocodozole Stimulated

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866) at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 39 kDa
Observed band size: 39 kDa

Exposure time: 8 minutes

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab45145 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
Immunoprecipitation - Anti-Aurora B antibody [EP1009Y] (ab45145)

Purified ab45145 at 1/20 dilution (1µg) immunoprecipitating Aurora B in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab45145 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab45145 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 39 kDa
OI-RD Scanning - Anti-Aurora B antibody [EP1009Y] (ab45145)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-Aurora B antibody [EP1009Y] (ab45145)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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