Anti-Bora antibody [EPR11610] (ab182149)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11610] to Bora
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Overview
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Product name
Anti-Bora antibody [EPR11610]
See all Bora primary antibodies -
Description
Rabbit monoclonal [EPR11610] to Bora -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIP HumanWB Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain, HeLa and Jurkat whole cell lysate (ab7899); HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11610 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Bora antibody [EPR11610] (ab182149) at 1/10000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 61 kDa
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Immunofluorescent analysis of HeLa cells (-20℃ Acetone-fixed) labeling Bora with ab182149 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 diution.
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Anti-Bora antibody [EPR11610] (ab182149) at 1/10000 dilution + Jurkat cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 61 kDa
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Western blot analysis of immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab182149 at 1/40 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
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