Anti-Aurora B (phospho S227) antibody [EPR20389] - BSA and Azide free (ab251522)
![Anti-Aurora B (phospho S227) antibody [EPR20389] - BSA and Azide free (ab251522)](https://www.abcam.com/ps/products/251/ab251522/Images/ab251522-447194-anti-aurora-b-phospho-s227-antibody-epr20389-bsa-and-azide-free-western-blot.jpg "Anti-Aurora B (phospho S227) antibody [EPR20389] - BSA and Azide free (ab251522)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20389] to Aurora B (phospho S227) - BSA and Azide free
- Suitable for: Dot blot, WB, IP, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Aurora B (phospho S227) antibody [EPR20389] - BSA and Azide free
See all Aurora B primary antibodies -
Description
Rabbit monoclonal [EPR20389] to Aurora B (phospho S227) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251522 is the carrier-free version of ab210706.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20389 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Aurora B (phospho S227) antibody [EPR20389] - BSA and Azide free (ab251522)All lanes : Anti-Aurora B (phospho S227) antibody [EPR20389] (ab210706) at 1/1000 dilution
Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa treated with 100 ng/ml nocodazole for 18 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDa
Exposure time: 3 minutesThis data was developed using ab210706, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry - Anti-Aurora B (phospho S227) antibody [EPR20389] - BSA and Azide free (ab251522)](https://www.abcam.com/ps/products/251/ab251522/Images/ab251522-4-anti-aurora-b-phospho-s227-antibody-epr20389-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-Aurora B (phospho S227) antibody [EPR20389] - BSA and Azide free (ab251522)This data was developed using ab210706, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Aurora B (phospho S227) with ab210706 at 1/10000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing midbody staining (arrow) on HeLa cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. -
![Immunoprecipitation - Anti-Aurora B (phospho S227) antibody [EPR20389] - BSA and Azide free (ab251522)](https://www.abcam.com/ps/products/251/ab251522/Images/ab251522-3-anti-aurora-b-phospho-s227-antibody-epr20389-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Aurora B (phospho S227) antibody [EPR20389] - BSA and Azide free (ab251522)This data was developed using ab210706, the same antibody clone in a different buffer formulation.
Aurora B (phospho S227) was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/ml nocodazole for 18 hours whole cell lysate with ab210706 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab210706 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: HeLa treated with 100 ng/ml nocodazole for 18 hours whole cell lysate 10μg (Input). Lane 2: ab210706 IP in HeLa treated with 100 ng/ml nocodazole for 18 hours whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab210706 in HeLa treated with 100 ng/ml nocodazole for 18 hours whole cell lysate. Blocking and dilution buffer: 5% NFDM/TBST. Exposure time: 30 seconds -
![Dot Blot - Anti-Aurora B (phospho S227) antibody [EPR20389] - BSA and Azide free (ab251522)](https://www.abcam.com/ps/products/251/ab251522/Images/ab251522-2-anti-aurora-b-phospho-s227-antibody-epr20389-bsa-and-azide-free-dot-blot.jpg)
Dot Blot - Anti-Aurora B (phospho S227) antibody [EPR20389] - BSA and Azide free (ab251522)This data was developed using ab210706, the same antibody clone in a different buffer formulation.
Dot blot analysis of Aurora B (phospho S227) labeled with ab210706 at 1/1000 dilution. Lane 1: Aurora B (phospho S227) peptide. Lane 2: Aurora B non-phospho peptide. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody. Blocking/Dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes -
![Anti-Aurora B (phospho S227) antibody [EPR20389] - BSA and Azide free (ab251522)](https://www.abcam.com/ps/products/251/ab251522/Images/ab251522-1-benefits-of-recombinant-antibodies.png)
Anti-Aurora B (phospho S227) antibody [EPR20389] - BSA and Azide free (ab251522)
