Formalin-fixed, paraffin-embedded human lung tissue stained for TTF1 with ab72876 at 1/50 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for TTF1 with ab72876 at 1/50 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human thyroid tissue stained for TTF1 with ab72876 at 1/50 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human lung tissue stained for TTF1 with ab72876 at 1/50 dilution in immunohistochemical analysis.

ab72876 at 1/50 dilution staining TTF1 in human lung by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

Overlay histogram showing A549 cells stained with ab72876 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab72876, 20µl/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.