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Immunology Adaptive Immunity T Cells CD

Anti-CD74 antibody [6D9] (ab223357)

Anti-CD74 antibody [6D9] (ab223357)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [6D9] to CD74
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-CD74 antibody [6D9]
    See all CD74 primary antibodies
  • Description

    Mouse monoclonal [6D9] to CD74
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD74 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P04233

  • Positive control

    • WB: Raji cell lysate. ICC/IF: HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    6D9
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [6D9] (ab223357)
    Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [6D9] (ab223357)

    4% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CD74 (green) using ab223357 at 1/100 dilution for 60 min at room temperature in ICC/IF, followed by Goat Anti-Mouse ATTO 488 at 1/200 dilution for 60 min at room temperature.

    (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223357 (D) Merge.

  • Western blot - Anti-CD74 antibody [6D9] (ab223357)
    Western blot - Anti-CD74 antibody [6D9] (ab223357)
    Anti-CD74 antibody [6D9] (ab223357) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) cell lysate at 15 µg

    Secondary
    Goat Anti-Mouse IgG HRP at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 34 kDa


    Exposure time: 5 minutes


    Blocking buffer: 5% skim milk in TBST

    Primary incubation: 2 hours at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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