Anti-CD74 antibody [6D9] (ab223357)
Key features and details
- Mouse monoclonal [6D9] to CD74
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD74 antibody [6D9]
See all CD74 primary antibodies -
Description
Mouse monoclonal [6D9] to CD74 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CD74 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P04233 -
Positive control
- WB: Raji cell lysate. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
6D9 -
Isotype
IgG1 -
Research areas
Images
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4% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CD74 (green) using ab223357 at 1/100 dilution for 60 min at room temperature in ICC/IF, followed by Goat Anti-Mouse ATTO 488 at 1/200 dilution for 60 min at room temperature.
(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223357 (D) Merge.
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Anti-CD74 antibody [6D9] (ab223357) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) cell lysate at 15 µg
Secondary
Goat Anti-Mouse IgG HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 34 kDa
Exposure time: 5 minutesBlocking buffer: 5% skim milk in TBST
Primary incubation: 2 hours at room temperature.