Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD74 antibody [In-1] (ab245692)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [In-1] to CD74
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD74 antibody [In-1]
    See all CD74 primary antibodies

  • Description

    Rabbit monoclonal [In-1] to CD74

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Tissue, cells or virus corresponding to CD74. Mouse lymphocytes of strain A injected into Wistar rats.

  • Positive control

    • ICC/IF: Mouse splenocytes. WB: Mouse spleen and lymph node lysates.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.02% Proclin 300
    Constituent: 99% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    In-1

  • Isotype

    IgG

  • Light chain type

    kappa

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [In-1] (ab245692)
    Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [In-1] (ab245692)

    PFA fixed, 0.15% Triton permeabilized mouse splenocytes labeling CD74 using ab245692 at 10 µg/ml for 1 hour (green) followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), in ICC/IF. The nuclear counter stain is DAPI (blue).

  • Western blot - Anti-CD74 antibody [In-1] (ab245692)
    Western blot - Anti-CD74 antibody [In-1] (ab245692)
    All lanes : Anti-CD74 antibody [In-1] (ab245692) at 0.0001 µg/ml (incubated for 1 hr)

    Lane 1 : Mouse spleen lysate (RIPA buffer)
    Lane 2 : Mouse lymph node lysate (RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 35 kDa



    10% SDS PAGE gel.

    Detected by chemiluminescence.

  • Anti-CD74 antibody [In-1] (ab245692)
    Anti-CD74 antibody [In-1] (ab245692)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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