Anti-CD74 antibody [In-1] (ab245692)
![Anti-CD74 antibody [In-1] (ab245692)](https://www.abcam.com/ps/products/245/ab245692/Images/ab245692-336961-anti-cd74-antibody-in-1-immunofluorescence.jpg "Anti-CD74 antibody [In-1] (ab245692)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [In-1] to CD74
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
Overview
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Product name
Anti-CD74 antibody [In-1]
See all CD74 primary antibodies -
Description
Rabbit monoclonal [In-1] to CD74 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseWB Mouse
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Immunogen
Tissue, cells or virus corresponding to CD74. Mouse lymphocytes of strain A injected into Wistar rats.
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Positive control
- ICC/IF: Mouse splenocytes. WB: Mouse spleen and lymph node lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
In-1 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [In-1] (ab245692)
PFA fixed, 0.15% Triton permeabilized mouse splenocytes labeling CD74 using ab245692 at 10 µg/ml for 1 hour (green) followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), in ICC/IF. The nuclear counter stain is DAPI (blue).
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![Western blot - Anti-CD74 antibody [In-1] (ab245692)](https://www.abcam.com/ps/products/245/ab245692/Images/ab245692-336960-anti-cd74-antibody-in-1-western-blot.jpg)
Western blot - Anti-CD74 antibody [In-1] (ab245692)All lanes : Anti-CD74 antibody [In-1] (ab245692) at 0.0001 µg/ml (incubated for 1 hr)
Lane 1 : Mouse spleen lysate (RIPA buffer)
Lane 2 : Mouse lymph node lysate (RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 35 kDa10% SDS PAGE gel.
Detected by chemiluminescence.
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![Anti-CD74 antibody [In-1] (ab245692)](https://www.abcam.com/ps/products/245/ab245692/Images/ab245692-3-benefits-of-recombinant-antibodies.png)
Anti-CD74 antibody [In-1] (ab245692)
