Anti-Histone H3.3 antibody (ab94817)
Key features and details
- Rabbit polyclonal to Histone H3.3
- Suitable for: WB
- Reacts with: Mouse, Cow, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Histone H3.3 antibody
See all Histone H3.3 primary antibodies -
Description
Rabbit polyclonal to Histone H3.3 -
Host species
Rabbit -
Specificity
This antibody was raised against a peptide derived from within residues 50 to the C-terminus of Human Histone H3.3. However, due to the extensive amount of homology between H3 variants, we expect this antibody to show some cross reactivity with H3.1 and H3.2. -
Tested Applications & Species
See all applications and species dataApplication Species WB CowHuman
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Immunogen
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Positive control
- This antibody gave a positive signal in the following whole cell lysates: HeLa; SAOS-2; Jurkat; NIH3T3.
Images
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Western blot - Anti-Histone H3.3 antibody (ab94817)All lanes : Anti-Histone H3.3 antibody (ab94817) at 1 µg/ml
Lane 1 : Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 4 : Histone H3.1 Recombinant Protein at 0.1 µg
Lane 5 : Histone H3.3 Recombinant Protein at 0.1 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
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Western blot - Anti-Histone H3.3 antibody (ab94817)All lanes : Anti-Histone H3.3 antibody (ab94817) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa Histone Preparation Nuclear Lysate
Lane 3 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Histone H3.3 peptide (ab129219) at 1 µg/ml
Lane 6 : HeLa Histone Preparation Nuclear Lysate with Histone H3.3 peptide (ab129219) at 1 µg/ml
Lane 7 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate with Histone H3.3 peptide (ab129219) at 1 µg/ml
Lane 8 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate with Histone H3.3 peptide (ab129219) at 1 µg/ml
Lane 9 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Histone H3.1 Peptide at 1 µg/ml
Lane 10 : HeLa Histone Preparation Nuclear Lysate with Histone H3.1 Peptide at 1 µg/ml
Lane 11 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate with Histone H3.1 Peptide at 1 µg/ml
Lane 12 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate with Histone H3.1 Peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Exposure time: 12 minutes
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Western blot - Anti-Histone H3.3 antibody (ab94817)All lanes : Anti-Histone H3.3 antibody (ab94817) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 50 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
