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Epigenetics and Nuclear Signaling Histones Variants

Anti-Histone H3.3 antibody (ab213677)

Anti-Histone H3.3 antibody (ab213677)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Histone H3.3
  • Suitable for: Flow Cyt, IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Histone H3.3 antibody
    See all Histone H3.3 primary antibodies
  • Description

    Rabbit polyclonal to Histone H3.3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide within Human Histone H3.3 aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P84243

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Rat brain, mouse intestine and human colon cancer tissues; K562 cells; Mouse splenocytes.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • Variants

Images

  • Western blot - Anti-Histone H3.3 antibody (ab213677)
    Western blot - Anti-Histone H3.3 antibody (ab213677)
    Anti-Histone H3.3 antibody (ab213677) at 1/300 dilution + K562 cell lysate

    Predicted band size: 15 kDa

  • Flow Cytometry - Anti-Histone H3.3 antibody (ab213677)
    Flow Cytometry - Anti-Histone H3.3 antibody (ab213677)

    Flow cytometric analysis of Mouse splenocytes labeling Histone H3.3 with ab213677 at 1/50 dilution for 30 minutes (green) followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody (ab213677)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody (ab213677)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon cancer tissue labeling Histone H3.3 with ab213677 at 1/400 dilution followed by conjugation to the secondary antibody and DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody (ab213677)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody (ab213677)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Mouse intestine tissue labeling Histone H3.3 with ab213677 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody (ab213677)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody (ab213677)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat brain tissue labeling Histone H3.3 with ab213677 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Histone H3.3 antibody (ab213677)

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    Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade (ab176840)

    Applications: ChIP, ChIP-seq, Dot, ICC/IF, IHC-P, WB

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    Alexa Fluor® 647 Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (ab237478)

    Applications: Flow Cyt, ICC/IF

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    Anti-Histone H3.3 antibody (ab94817)

    Applications: WB

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    Anti-Histone H3.3 antibody [EPR17899] - BSA and Azide free (ab208690)

    Applications: ChIP, ChIP-seq, Dot, ICC/IF, IHC-P, WB

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    Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (ab92628)

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    Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - BSA and Azide free (ab183300)

    Applications: Dot, Flow Cyt, ICC/IF, IHC-P, WB

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    Alexa Fluor® 488 Anti-Histone H3.3 (phospho S31) antibody [EPR1873] (ab237477)

    Applications: Flow Cyt, ICC/IF

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