Anti-TNP1 antibody (ab73135)
Key features and details
- Rabbit polyclonal to TNP1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TNP1 antibody
See all TNP1 primary antibodies -
Description
Rabbit polyclonal to TNP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide corresponding to Human TNP1 aa 1 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab104948) -
Positive control
- This antibody gave a positive signal in Human normal testis FFPE section.
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General notes
Previously labelled as Transition Protein 1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-P HumanAll applications MouseRatDogPigApplication Abreviews Notes IHC-P (1) Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.Notes IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.Target
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Function
In the elongating spermatids of mammals, the conversion of nucleosomal chromatin to the compact, non-nucleosomal form found in the sperm nucleus is associated with the appearance of a small set of basic chromosomal transition proteins. -
Tissue specificity
Testis. -
Sequence similarities
Belongs to the nuclear transition protein 1 family. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 7141 Human
- Entrez Gene: 21958 Mouse
- Entrez Gene: 100513764 Pig
- Entrez Gene: 24839 Rat
- Omim: 190231 Human
- SwissProt: P09430 Human
- SwissProt: P10856 Mouse
- SwissProt: P17306 Pig
see all -
Alternative names
- Spermatid nuclear transition protein 1 antibody
- Spermatid nuclear transition protein1 antibody
- STP 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNP1 antibody (ab73135)
IHC image of TNP1 staining in Human normal testis FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73135, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
Datasheets and documents
References (6)
ab73135 has been referenced in 6 publications.
- Wang S et al. BRG1 Is Dispensable for Sertoli Cell Development and Functions in Mice. Int J Mol Sci 21:N/A (2020). PubMed: 32575410
- Zhou H et al. The testicular soma of Tsc22d3 knockout mice supports spermatogenesis and germline transmission from spermatogonial stem cell lines upon transplantation. Genesis 57:e23295 (2019). PubMed: 31001916
- Han H et al. Testicular Characteristics and the Block to Spermatogenesis in Mature Hinny. Asian-Australas J Anim Sci 29:793-800 (2016). IF . PubMed: 26954128
- Schneider S et al. Re-visiting the Protamine-2 locus: deletion, but not haploinsufficiency, renders male mice infertile. Sci Rep 6:36764 (2016). PubMed: 27833122
- Yassine S et al. Dpy19l2-deficient globozoospermic sperm display altered genome packaging and DNA damage that compromises the initiation of embryo development. Mol Hum Reprod 21:169-85 (2015). PubMed: 25354700
- Yuen BT et al. Histone H3.3 regulates dynamic chromatin states during spermatogenesis. Development 141:3483-94 (2014). PubMed: 25142466
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNP1 antibody (ab73135)
IHC image of TNP1 staining in Human normal testis FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73135, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
