Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TNP1 antibody (ab73135)

Key features and details

  • Rabbit polyclonal to TNP1
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-TNP1 antibody
    See all TNP1 primary antibodies

  • Description

    Rabbit polyclonal to TNP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human TNP1 aa 1 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab104948)

  • Positive control

    • This antibody gave a positive signal in Human normal testis FFPE section.

  • General notes

    Previously labelled as Transition Protein 1.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab73135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
All applications
Mouse
Rat
Dog
Pig
Application Abreviews Notes
IHC-P (1)
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNP1 antibody (ab73135)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNP1 antibody (ab73135)

    IHC image of TNP1 staining in Human normal testis FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73135, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

References (6)

Publishing research using ab73135? Please let us know so that we can cite the reference in this datasheet.

ab73135 has been referenced in 6 publications.

  • Wang S  et al. BRG1 Is Dispensable for Sertoli Cell Development and Functions in Mice. Int J Mol Sci 21:N/A (2020). PubMed: 32575410
  • Zhou H  et al. The testicular soma of Tsc22d3 knockout mice supports spermatogenesis and germline transmission from spermatogonial stem cell lines upon transplantation. Genesis 57:e23295 (2019). PubMed: 31001916
  • Han H  et al. Testicular Characteristics and the Block to Spermatogenesis in Mature Hinny. Asian-Australas J Anim Sci 29:793-800 (2016). IF . PubMed: 26954128
  • Schneider S  et al. Re-visiting the Protamine-2 locus: deletion, but not haploinsufficiency, renders male mice infertile. Sci Rep 6:36764 (2016). PubMed: 27833122
  • Yassine S  et al. Dpy19l2-deficient globozoospermic sperm display altered genome packaging and DNA damage that compromises the initiation of embryo development. Mol Hum Reprod 21:169-85 (2015). PubMed: 25354700
  • Yuen BT  et al. Histone H3.3 regulates dynamic chromatin states during spermatogenesis. Development 141:3483-94 (2014). PubMed: 25142466

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNP1 antibody (ab73135)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNP1 antibody (ab73135)

    IHC image of TNP1 staining in Human normal testis FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73135, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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