Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18090] to Histone H2A.Z - ChIP Grade
  • Suitable for: ChIP, PepArr, ICC/IF, WB, IHC-P, ChIP-sequencing
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade
    See all Histone H2A.Z primary antibodies

  • Description

    Rabbit monoclonal [EPR18090] to Histone H2A.Z - ChIP Grade

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP
    Human
    ChIP-seq
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    PepArr
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and NIH/3T3 whole cell lysates. IHC-P: Human, mouse and rat colon tissues. ICC/IF: HeLa cells. ChIP: HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18090

  • Isotype

    IgG

  • Research areas

Images

  • ChIP-sequencing - Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314)
    ChIP-sequencing - Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314)

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 µg of chromatin and 4 µg of ab223152 [EPR18090]. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 10 million sequence tags. The image shows binding across a region of chromosome 1 (RLF gene). ChIP-Seq validation performed by Active Motif, Carlsbad, CA

    This image was generated using ab223152. The same clone but a different buffer formulation. 

  • ChIP - Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314)
    ChIP - Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314)

    Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab188314 (blue), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H2A.Z with ab188314 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab188314 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H2A.Z with ab188314 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on Human colon tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Histone H2A.Z with ab188314 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on mouse colon tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314)

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Histone H2A.Z with ab188314 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on rat colon tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Peptide Array - Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314)
    Peptide Array - Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314)

    ab188314 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.

  • Western blot - Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314)
    Western blot - Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314)
    Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314) at 1/10000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314)
    Western blot - Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314)
    Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314) at 1/10000 dilution + NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14 kDa


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314)
    Anti-Histone H2A.Z antibody [EPR18090] - ChIP Grade (ab188314)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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