Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - BSA and Azide free (ab208691)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6171(2)(B)] to Histone H2A.Z - BSA and Azide free
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF, ChIP
Reacts with: Mouse, Rat, Human

Product name

Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - BSA and Azide free
See all Histone H2A.Z primary antibodies
Description

Rabbit monoclonal [EPR6171(2)(B)] to Histone H2A.Z - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
ChIP	Human
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human lung carcinoma and colon tissue. ICC/IF: HepG2 cells. Flow Cyt: HeLa cells.
General notes
Ab208691 is the carrier-free version of ab150402. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab208691 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6171(2)(B)
Isotype

IgG
Research areas

ChIP - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - BSA and Azide free (ab208691)

Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol*. Cells were fixed with formaldehyde for 10min.
The ChIP was performed with 25 µg of chromatin, 2 µg of ab150402 (red), and 20 µl of Protein A/G sepharose beads. 2 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
Primers and probes are located in the first kb of the transcribed region.
*http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150402).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - BSA and Azide free (ab208691)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast tissue sections labeling Histone H2A.Z with purified ab150402 at 1/2000 dilution (1.09 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150402).
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - BSA and Azide free (ab208691)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Histone H2A.Z with purified ab150402 at 1:200 dilution (10 µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150402).
Flow Cytometry - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - BSA and Azide free (ab208691)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Histone H2A.Z (red) with purified ab150402 at a 1/2500 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150402).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - BSA and Azide free (ab208691)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue sections labeling Histone H2A.Z with purified ab150402 at 1/2000 dilution (1.09 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150402).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - BSA and Azide free (ab208691)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling Histone H2A.Z with purified ab150402 at 1/2000 dilution (1.09 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150402).
ChIP - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - BSA and Azide free (ab208691)

Chromatin was prepared from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab150402 (blue), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach). Primers and probes are located in the first kb of the transcribed region.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150402).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - BSA and Azide free (ab208691)

Immunohistochemical analysis of paraffin embedded human ovarian carcinoma tissue using ab150402 (unpurified) showing positive staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150402).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - BSA and Azide free (ab208691)

Immunohistochemical analysis of paraffin embedded human prostate hyperplasia tissue using ab150402 (unpurified) showing positive staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150402).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - BSA and Azide free (ab208691)

Immunohistochemical analysis of paraffin embedded normal human kidney tissue using ab150402 (unpurified) showing positive staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150402).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - BSA and Azide free (ab208691)

Immunohistochemical analysis of paraffin embedded human cervical carcinoma tissue using ab150402 (unpurified) showing positive staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150402).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - BSA and Azide free (ab208691)

Immunofluorescent analysis of HepG2 (Human liver hepatocellular carcinoma cell line cells labeling Histone H2A.Z with ab150402 (unpurified) at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150402).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - BSA and Azide free (ab208691)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling Histone H2A.Z with ab150402 (unpurified) at 1/250 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150402).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - BSA and Azide free (ab208691)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Histone H2A.Z with ab150402 (unpurified) at 1/250 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150402).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - BSA and Azide free (ab208691)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com