Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - BSA and Azide free (ab183300)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1873] to Histone H3.3 (phospho S31) - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, WB, Flow Cyt, Dot blot
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - BSA and Azide free
    See all Histone H3.3 primary antibodies

  • Description

    Rabbit monoclonal [EPR1873] to Histone H3.3 (phospho S31) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, Flow Cyt, Dot blotmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab183300 is the carrier-free version of ab92628. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab183300 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR1873

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - BSA and Azide free (ab183300)
    Flow Cytometry - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - BSA and Azide free (ab183300)

    Flow Cytometry analysis of Hela (human cervix adenocarcinoma) cells treated with 100 ng/ml Nocodazole for 18 hours, labeling Histone H3.3 with purified ab92628 at 1/150 dilution (10µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. Untreated control - Hela (human cervix adenocarcinoma) cells untreated with 100 ng/ml Nocodazole for 18 hours (Green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92628).

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - BSA and Azide free (ab183300)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - BSA and Azide free (ab183300)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% tritonX-100 HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, LP starved and non-starved, labeling anti-Histone H3.3 (phospho S31) with Ab92628 at 1/500 dilution followed by Goat anti-Rabbit secondary IgG AlexaFluor®488 (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on M phase of HeLa cells, then the signal decreased after LP treatment.

    For the pan antibody, there was no great difference after LP treatment. The data showed mostly nuclear staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92628).

  • Dot Blot - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - BSA and Azide free (ab183300)
    Dot Blot - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - BSA and Azide free (ab183300)

    Dot Blot analysis of Lane 1: Histone H3.3 (pS31) phospho peptide and Lane 2: Histone H3.3 non-phospho peptide labeling Histone H3.3 (phospho S31) with ab92628 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. 

    ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 10 seconds.

     

     

     

     

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92628).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - BSA and Azide free (ab183300)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - BSA and Azide free (ab183300)

    Paraffin-embedded human cervical carcinoma labelled with ab92628 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92628).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - BSA and Azide free (ab183300)
    Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - BSA and Azide free (ab183300)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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