Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - BSA and Azide free (ab183300)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1873] to Histone H3.3 (phospho S31) - BSA and Azide free
Suitable for: ICC/IF, IHC-P, WB, Flow Cyt, Dot blot
Reacts with: Mouse, Rat, Human

Product name

Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - BSA and Azide free
See all Histone H3.3 primary antibodies
Description

Rabbit monoclonal [EPR1873] to Histone H3.3 (phospho S31) - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, IHC-P, WB, Flow Cyt, Dot blotmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab183300 is the carrier-free version of ab92628. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab183300 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR1873
Isotype

IgG
Research areas

Flow Cytometry - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - BSA and Azide free (ab183300)

Flow Cytometry analysis of Hela (human cervix adenocarcinoma) cells treated with 100 ng/ml Nocodazole for 18 hours, labeling Histone H3.3 with purified ab92628 at 1/150 dilution (10µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti-rabbit IgG (Alexa Fluor^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. Untreated control - Hela (human cervix adenocarcinoma) cells untreated with 100 ng/ml Nocodazole for 18 hours (Green).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92628).
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - BSA and Azide free (ab183300)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% tritonX-100 HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, LP starved and non-starved, labeling anti-Histone H3.3 (phospho S31) with Ab92628 at 1/500 dilution followed by Goat anti-Rabbit secondary IgG AlexaFluor®488 (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing nuclear staining on M phase of HeLa cells, then the signal decreased after LP treatment.

For the pan antibody, there was no great difference after LP treatment. The data showed mostly nuclear staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92628).
Dot Blot - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - BSA and Azide free (ab183300)

Dot Blot analysis of Lane 1: Histone H3.3 (pS31) phospho peptide and Lane 2: Histone H3.3 non-phospho peptide labeling Histone H3.3 (phospho S31) with ab92628 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer.

ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 10 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92628).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - BSA and Azide free (ab183300)

Paraffin-embedded human cervical carcinoma labelled with ab92628 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92628).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Histone H3.3 (phospho S31) antibody [EPR1873] - BSA and Azide free (ab183300)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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