Anti-Histone H3.3 antibody (ab183902)
Key features and details
- Rabbit polyclonal to Histone H3.3
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3.3 antibody
See all Histone H3.3 primary antibodies -
Description
Rabbit polyclonal to Histone H3.3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig, Xenopus laevis, Xenopus tropicalis
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Immunogen
Recombinant fragment within Human Histone H3.3 aa 1-136. The exact sequence is proprietary.
Database link: P84243 -
Positive control
- Saos2 xenograft and mouse intestine tissues. Jurkat, Raji, K562, THP1, HL60, NCI-H929, mouse JC, mouse BCL-1, mouse NIH 3T3 and Rat PC12 whole cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H3.3 antibody (ab183902)All lanes : Anti-Histone H3.3 antibody (ab183902) at 1/10000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : THP1 whole cell lysate
Lane 5 : HL60 whole cell lysate
Lane 6 : NCI-H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 15 kDa
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Western blot - Anti-Histone H3.3 antibody (ab183902)All lanes : Anti-Histone H3.3 antibody (ab183902) at 1/10000 dilution
Lane 1 : NIH 3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL-1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 15 kDa
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Western blot - Anti-Histone H3.3 antibody (ab183902)Anti-Histone H3.3 antibody (ab183902) at 1/10000 dilution + PC12 whole cell lysate at 30 µg
Predicted band size: 15 kDa15% SDS PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody (ab183902)Immunohistochemical analysis of paraffin-embedded Saos2 (human) xenograft tissue, labeling Histone H3.3 with ab183902 at a 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody (ab183902)Immunohistochemical analysis of paraffin-embedded mouse intestine tissue, labeling Histone H3.3 with ab183902 at a 1/500 dilution.
