Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694)

Key features and details

  • Rabbit monoclonal [EP800Y] to CENPA - Centromere Marker
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-CENPA antibody [EP800Y] - Centromere Marker
    See all CENPA primary antibodies

  • Description

    Rabbit monoclonal [EP800Y] to CENPA - Centromere Marker

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human CENPA aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa whole cell lysate (ab150035), Human colon

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694)
    Western blot - Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694)
    All lanes : Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694) at 1/1000 dilution (purified)

    Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
    Lane 2 : Human melanoma
    Lane 3 : A375 (human malignant melanoma) whole cell lysate
    Lane 4 : HCT-116 (human colorectal carcinoma) whole cell lysate
    Lane 5 : Human placenta lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 16 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer 5% NFDM/TBST
  • Western blot - Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694)
    Western blot - Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694)
    Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694) at 1/20000 dilution (unpurified) + HeLa cell lysate (10ug/lane)

    Predicted band size: 16 kDa
    Observed band size: 18 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694)

    Immunohistochemical analysis of paraffin embedded human colon tissue section labelling CENPA with purified ab45694 at dilution of 1/250. The secondary antibody used was HRP-conjugated Goat Anti-Rabbit IgG H&L (ab97051) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694)

    IHC image of ab45694 staining CENPA in Human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with unpurified ab45694, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694)

    Immunohistochemical analysis of formalin fixed, paraffin embedded colonic adenocarcinoma tissue section labelling CENPA with unpurified ab45694.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694)

    Immunohistochemical analysis of formalin fixed, paraffin embedded Lymphoma tissue section labelling CENPA with unpurified ab45694.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694)

    Immunohistochemical analysis of formalin fixed, paraffin embedded prostatic carcinoma tissue section labelling CENPA with unpurified ab45694.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694)

    Immunohistochemical analysis of formalin fixed, paraffin embedded lung carcinoma tissue section labelling CENPA with unpurified ab45694.

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