Anti-Methylglyoxal antibody [9F11] (ab243074)
![Anti-Methylglyoxal antibody [9F11] (ab243074)](https://www.abcam.com/ps/products/243/ab243074/Images/ab243074-332322-anti-methylglyoxal-antibody-9f11-western-blot.png "Anti-Methylglyoxal antibody [9F11] (ab243074)")
Key features and details
- Mouse monoclonal [9F11] to Methylglyoxal
- Suitable for: WB, Flow Cyt
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-Methylglyoxal antibody [9F11] -
Description
Mouse monoclonal [9F11] to Methylglyoxal -
Host species
Mouse -
Specificity
Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdeyhde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal.
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Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Methylglyoxal conjugated to keyhole limpet haemocyanin.
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Positive control
- WB: Methylglyoxal-BSA Conjugate. Flow cyt: SH-SY5Y cells (treated with hydrogen peroxide).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
9F11 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Methylglyoxal antibody [9F11] (ab243074)All lanes : Anti-Methylglyoxal antibody [9F11] (ab243074) at 1/1000 dilution
Lane 1 : Methylglyoxal-BSA
Lane 2 : BSA
Lysates/proteins at 0.5 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG: HRP at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 5 minutesPrimary incubation for 2 hours at room temperature.
Secondary incubation for 1 hour at room temperature.
Block: 5% Skimmed milk in TBST.
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![Flow Cytometry - Anti-Methylglyoxal antibody [9F11] (ab243074)](https://www.abcam.com/ps/products/243/ab243074/Images/ab243074-332323-anti-methylglyoxal-antibody-9f11-flow-cytometry.png)
Flow Cytometry - Anti-Methylglyoxal antibody [9F11] (ab243074)Flow cytometric analysis of 90% methanol-fixed SH-SY5Y (human neuroblastoma cell line from bone marrow) cells (+ 250 μM hydrogen peroxide treatment for 24 hours) labeling Methylglyoxal with ab243074 at 1/50 dilution for 30 minutes on ice (solid line) compared with an Isotype control IgG (dashed line). A Goat Anti-Mouse: PE at 1/100 dilution was used as the secondary antibody (20 mins at RT).
