Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human RUNX2 Antibody Pair - BSA and Azide free (ab253543)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

Overview

  • Product name

    Human RUNX2 Antibody Pair - BSA and Azide free
    See all RUNX2 kits

  • Assay type

    ELISA set

  • Range

    7.813 pg/ml - 2000 pg/ml

  • Species reactivity

    Reacts with: Human

  • Product overview


    • Human RUNX2 Antibody Pair is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human RUNX2 in sandwich ELISAs and many other pair-based applications.

    • The pair can be used in variety of assays and platforms including but not limited to:

    •      - Sandwich ELISA

    •      - FRET/TR-FRET/HTR

    •      - Meso Scale Discovery® (MSD®)

    •      - Luminex® and bead-based assays

    •      - AlphaLISA®/AlphaScreen®

    •      - DELFIA® immunoassays

    •      - Simoa® and Single Molecule Counting (SMC™) immunoassays

    •      - Multiplex

    • Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

    •      - Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.

    •      - Concentration of ~1 mg/ml as measured by the protein A280 method.

    • Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
    • We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

    • Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

    • Please note:

    • The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

    • The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

    • We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

    •  Antibody properties:

    •  Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

    •  Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

    •  Concentration: ~1 mg/ml

    •  Storage buffer: 100% PBS

    •  Form: Liquid

    •  Isotype: IgG

    •  Recombinant monoclonal antibodies offer several advantages including:

    •      - High batch-to-batch consistency and reproducibility

    •      - Improved sensitivity and specificity

    •      - Long-term security of supply

    •      - Animal-free production

    • For more information see here.

    • Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    • Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

    • Luminex is a trademark of Luminex Corporation, registered in the US and other countries.

    • AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

    • Simoa is a registered trademark of Quanterix, Inc.

    • SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.

  • Carrier free

    Yes
  • Components Identifier 10 x 96 tests
    Human RUNX2 Capture Antibody (unconjugated) —
    Human RUNX2 Detector Antibody (unconjugated) —

  • Research areas

  • Function

    Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters (By similarity). Inhibits MYST4-dependent transcriptional activation.

  • Tissue specificity

    Specifically expressed in osteoblasts.

  • Involvement in disease

    Defects in RUNX2 are the cause of cleidocranial dysplasia (CLCD) [MIM:119600]; also known as cleidocranial dysostosis (CCD). CLCD is an autosomal dominant skeletal disorder with high penetrance and variable expressivity. It is due to defective endochondral and intramembranous bone formation. Typical features include hypoplasia/aplasia of clavicles, patent fontanelles, wormian bones (additional cranial plates caused by abnormal ossification of the calvaria), supernumerary teeth, short stature, and other skeletal changes. In some cases defects in RUNX2 are exclusively associated with dental anomalies.

  • Sequence similarities

    Contains 1 Runt domain.

  • Domain

    A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes and contains the phosphorylation sites.

  • Post-translational
    modifications

    Phosphorylated; probably by MAP kinases (MAPK) (By similarity). Isoform 3 is phosphorylated on Ser-340.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Alternative names

    • Acute myeloid leukemia 3 protein
    • Alpha subunit 1
    • AML3
    • CBF alpha 1
    • CBF-alpha-1
    • CBFA1
    • CCD
    • CCD1
    • Cleidocranial dysplasia 1
    • Core binding factor
    • Core binding factor runt domain alpha subunit 1
    • Core binding factor subunit alpha 1
    • Core-binding factor subunit alpha-1
    • MGC120022
    • MGC120023
    • Oncogene AML 3
    • Oncogene AML-3
    • OSF 2
    • OSF-2
    • OSF2
    • Osteoblast specific transcription factor 2
    • Osteoblast-specific transcription factor 2
    • OTTHUMP00000016533
    • PEA2 alpha A
    • PEA2-alpha A
    • PEA2aA
    • PEBP2 alpha A
    • PEBP2-alpha A
    • PEBP2A1
    • PEBP2A2
    • PEBP2aA
    • PEBP2aA1
    • Polyomavirus enhancer binding protein 2 alpha A subunit
    • Polyomavirus enhancer-binding protein 2 alpha A subunit
    • Runt domain
    • Runt related transcription factor 2
    • Runt-related transcription factor 2
    • RUNX2
    • RUNX2_HUMAN
    • SL3 3 enhancer factor 1 alpha A subunit
    • SL3-3 enhancer factor 1 alpha A subunit
    • SL3/AKV core binding factor alpha A subunit
    • SL3/AKV core-binding factor alpha A subunit
    see all

  • Database links

    Images

    • Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253543)
      Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253543)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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