Anti-EDC3 antibody (ab168851)
Key features and details
- Rabbit polyclonal to EDC3
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-EDC3 antibody -
Description
Rabbit polyclonal to EDC3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 25-75 of Human EDC3 (NP_079359.2).
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Positive control
- 293T, HeLa, Jurkat, mouse TCMK-1 and mouse NIH3T3 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab168851 was affinity purified using an epitope specific to EDC3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EDC3 antibody (ab168851)All lanes : Anti-EDC3 antibody (ab168851) at 0.1 µg
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 56 kDa
Exposure time: 10 seconds
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Immunoprecipitation - Anti-EDC3 antibody (ab168851)Detection of EDC3 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168851 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 10 seconds