Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µg 307 566 ₸ 10 µg 90 460 ₸

Order now and get it on Wednesday March 03, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [R10Z8E9] to IgG
Suitable for: WB, ELISA
Reacts with: Human

Product name

Anti-IgG antibody [R10Z8E9]
See all IgG primary antibodies
Description

Mouse monoclonal [R10Z8E9] to IgG
Host species

Mouse
Tested applications

Suitable for: WB, ELISAmore details
Unsuitable for: IHC-P
Species reactivity

Reacts with: Human
Immunogen

Full length protein. This information is considered to be commercially sensitive.
Positive control
- ELISA: Human IgG1. WB: Human serum lysate.
General notes
This product has switched from a hybridoma to recombinant production method on 21st September 2020.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.04% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

R10Z8E9
Isotype

IgG1
Research areas

Western blot - Anti-IgG antibody [R10Z8E9] (ab124055)

Anti-IgG antibody [R10Z8E9] (ab124055) at 1/1000 dilution + Human serum lysate at 10 µg

Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366)

Predicted band size: 36 kDa

Exposure time: 3 minutes

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Expected MW: 50 kDa.
ELISA - Anti-IgG antibody [R10Z8E9] (ab124055)

50ng Human IgG1 (50μL at 1000ng/mL) was coated onto 96-wells. Serial dilutions (0, 0.001, 0.005, 0.020, 0.078, 0.313, 1.25, 5μg/mL) of ab124055 (50μL) were incubated with Human IgG1 for 60 minutes by shaking. This was followed by adding goat anti-mouse IgG, (H+L), phosphatase-conjugated secondary antibody 1/1000 diluted (50μL) into each well and incubating for another 40 minutes. After washing, 50 μL of PNPP was added and incubated for 13 minutes without shaking for color development. OD was read at 405nm within 5 minutes.
Anti-IgG antibody [R10Z8E9] (ab124055)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com