IHC image of formalin-fixed paraffin-embedded normal mouse brain sections tested on a Leica BOND™. Sections were pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The sections were then incubated with antibody (ab185065, Rabbit monoclonal to sodium potassium ATPase, at 1/50 dilution) or isotype control (ab199507, Rabbit IgG, at 1/50 dilution) for 15 mins at room temperature. DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX.

The background control image is taken from an identical assay without primary antibody or isotype control.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC image of formalin-fixed paraffin-embedded normal mouse spleen sections tested on a Leica BOND™. Sections were pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The sections were then incubated with antibody (ab185067, Rabbit monoclonal to alpha tubulin, at 1/100 dilution) or isotype control (ab199507, Rabbit IgG, at 1/50 dilution) for 15 mins at room temperature. DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX.

The background control image is taken from an identical assay without primary antibody or isotype control.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC image of formalin-fixed paraffin-embedded normal rat brain sections tested on a Leica BOND™. Sections were pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The sections were then incubated with antibody (ab185065, Rabbit monoclonal to sodium potassium ATPase, at 1/50 dilution) or isotype control (ab199507, Rabbit IgG, at 1/50 dilution) for 15 mins at room temperature. DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX.

The background control image is taken from an identical assay without primary antibody or isotype control.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC image of formalin-fixed paraffin-embedded normal rat spleen sections tested on a Leica BOND™. Sections were pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The sections were then incubated with antibody (ab185067, Rabbit monoclonal to alpha tubulin, at 1/100 dilution) or isotype control (ab199507, Rabbit IgG, at 1/50 dilution) for 15 mins at room temperature. DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX.

The background control image is taken from an identical assay without primary antibody or isotype control.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC image of formalin-fixed paraffin-embedded normal human cerebral cortex sections* tested on a Leica BOND™. Sections were pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The sections were then incubated with antibody (ab185065, Rabbit monoclonal to sodium potassium ATPase, at 1/50 dilution) or isotype control (ab199507, Rabbit IgG, at 1/50 dilution) for 15 mins at room temperature. DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX.

The background control image is taken from an identical assay without primary antibody or isotype control.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

IHC image of formalin-fixed paraffin-embedded normal human spleen sections* tested on a Leica BOND™. Sections were pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The sections were then incubated with antibody (ab185067, Rabbit monoclonal to alpha tubulin, at 1/100 dilution) or isotype control (ab199507, Rabbit IgG, at 1/50 dilution) for 15 mins at room temperature. DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX.

The background control image is taken from an identical assay without primary antibody or isotype control.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.