Alexa Fluor® 594 Mouse IgG2a, kappa monoclonal [10D7A7B2] - Isotype Control (ab178001)
Overview
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Product name
Alexa Fluor® 594 Mouse IgG2a, kappa monoclonal [10D7A7B2] - Isotype Control
See all IgG isotype controls -
Conjugation
Alexa Fluor® 594. Ex: 590nm, Em: 617nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
General notes
This IgG2a kappa isotype control monoclonal antibody was raised in mouse against a yeast-specific protein, V-ATPase subunit (the product of VPH1 gene). It is a suitable isotype control for mammalian species.
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and conjugation.
Product was previously marketed under the MitoSciences sub-brand.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
10D7A7B2 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
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Cellular localization
Secreted -
Alternative names
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
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Images
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Immunocytochemistry data for ab178001. All panels show HeLa cells stained with DAPI as well as; a no primary control (A), ab178001 at 1 μg/mL (B), an Alexa 594 anti-SDHA conjugated antibody (ab170172) at 1 μg/mL (C).
Cells were PFA fixed and stored in 0.02%azide/PBS in 4°C. Cells were blocked with 10%GS for 2 hours, primary was diluted in 10%GS and incubated for 2 hours, cells were washed 3x with 1%GS and DAPI was added for 10 minutes in 1%GS.