Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15107(B)] to ACADVL/VLCAD
  • Suitable for: IP, WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ACADVL/VLCAD antibody [EPR15107(B)]
    See all ACADVL/VLCAD primary antibodies

  • Description

    Rabbit monoclonal [EPR15107(B)] to ACADVL/VLCAD

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HepG2, A431 and 293 cell lysates. IHC-P: Human kidney tissue and colon adenocarcinoma tissue. WB: HeLa cells.

  • General notes

     

     

    Previously labelled as ACADVL. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
    Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
    All lanes : Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : ACADVL knockout HEK293T cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 70 kDa
    Observed band size: 70 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab188872 observed at 70 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab188872 Anti-ACADVL/VLCAD antibody [EPR15107(B)] was shown to specifically react with ACADVL/VLCAD in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266484 (knockout cell lysate ab257332) was used. Wild-type and ACADVL/VLCAD knockout samples were subjected to SDS-PAGE. ab188872 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
    Flow Cytometry - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)

    Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling ACADVL/VLCAD with ab188872 at 1/160 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)

    Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labeling ACADVL/VLCAD with ab188872 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ACADVL/VLCAD with ab188872 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
    Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
    Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872) at 1/1000 dilution + 293 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 70 kDa

  • Immunoprecipitation - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
    Immunoprecipitation - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)

    Western blot analysis of immunoprecipitation pellet from A431 lysate immunoprecipitated using ab188872 at 1/50 dilution.

    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

  • Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
    Western blot - Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
    All lanes : Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 70 kDa

  • Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)
    Anti-ACADVL/VLCAD antibody [EPR15107(B)] (ab188872)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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