Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bak antibody [AT38E2] (ab104124)

Key features and details

  • Mouse monoclonal [AT38E2] to Bak
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Bak antibody [AT38E2]
    See all Bak primary antibodies

  • Description

    Mouse monoclonal [AT38E2] to Bak

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to amino acids 29-187 of Human Bak, purified from E. coli.

  • Positive control

    • WB: Mouse lung lysate, 293T, HeLa, A431, A549, Jurkat, MCF7, and PC3 cell lysates ICC/IF: HeLa cells. Flow Cyt: HeLa cells.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    AT38E2

  • Isotype

    IgG2a

  • Light chain type

    kappa

  • Research areas

Images

  • Western blot - Anti-Bak antibody [AT38E2] (ab104124)
    Western blot - Anti-Bak antibody [AT38E2] (ab104124)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: BAK knockout HAP1 whole cell lysate (20 µg)

    Lanes 1 - 2: Merged signal (red and green). Green - ab104124 observed at 0 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab104124 was found to be non-specific when BAK knockout samples were used. Wild-type and BAK knockout samples were subjected to SDS-PAGE. Ab104124 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/250 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This image was produced using the ascites version of this antibody. 

  • Flow Cytometry - Anti-Bak antibody [AT38E2] (ab104124)
    Flow Cytometry - Anti-Bak antibody [AT38E2] (ab104124)

    Flow cytometry analysis of BAK in HeLa cells. The cell was stained with ab104124 at 2-5µg for 1x106 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).

  • Immunocytochemistry/ Immunofluorescence - Anti-Bak antibody [AT38E2] (ab104124)
    Immunocytochemistry/ Immunofluorescence - Anti-Bak antibody [AT38E2] (ab104124)
    Immunocytochemistry/ Immunofluorescence analysis of BAK in HeLa cells. The cell was stained with ab104124 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
  • Western blot - Anti-Bak antibody [AT38E2] (ab104124)
    Western blot - Anti-Bak antibody [AT38E2] (ab104124)
    All lanes : Anti-Bak antibody [AT38E2] (ab104124) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : A549 cell lysate
    Lane 5 : Jurkat cell lysate
    Lane 6 : MCF7 cell lysate
    Lane 7 : PC3 cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : goat anti-mouse secondary antibody conjugated to HRP

    Predicted band size: 23 kDa

  • Western blot - Anti-Bak antibody [AT38E2] (ab104124)
    Western blot - Anti-Bak antibody [AT38E2] (ab104124)
    Anti-Bak antibody [AT38E2] (ab104124) at 1/500 dilution + Mouse lung lyasate at 35 µg

    Secondary
    Goat anti-mouse HRP conjugate

    Developed using the ECL technique.

    Predicted band size: 23 kDa



    This image was produced using the ascites version of this antibody. 

  • Immunocytochemistry/ Immunofluorescence - Anti-Bak antibody [AT38E2] (ab104124)
    Immunocytochemistry/ Immunofluorescence - Anti-Bak antibody [AT38E2] (ab104124)

    Immunofluorescence staining of Bak in HeLa cells using Hoechst 3342 (Blue) for nucleus staining and ab104124 antibody (1/500) with Texas Red (Red).

    This image was produced using the ascites version of this antibody. 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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