Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µg 278 083 ₸

Order now and get it on Friday March 05, 2021

Key features and details

Mouse monoclonal [AT38E2] to Bak
Suitable for: Flow Cyt, WB, ICC/IF
Reacts with: Mouse, Human
Isotype: IgG2a

Product name

Anti-Bak antibody [AT38E2]
See all Bak primary antibodies
Description

Mouse monoclonal [AT38E2] to Bak
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to amino acids 29-187 of Human Bak, purified from E. coli.
Positive control
- WB: Mouse lung lysate, 293T, HeLa, A431, A549, Jurkat, MCF7, and PC3 cell lysates ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
General notes

This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

AT38E2
Isotype

IgG2a
Light chain type

kappa
Research areas

Western blot - Anti-Bak antibody [AT38E2] (ab104124)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: BAK knockout HAP1 whole cell lysate (20 µg)

Lanes 1 - 2: Merged signal (red and green). Green - ab104124 observed at 0 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab104124 was found to be non-specific when BAK knockout samples were used. Wild-type and BAK knockout samples were subjected to SDS-PAGE. Ab104124 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/250 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

This image was produced using the ascites version of this antibody.
Flow Cytometry - Anti-Bak antibody [AT38E2] (ab104124)

Flow cytometry analysis of BAK in HeLa cells. The cell was stained with ab104124 at 2-5µg for 1x10⁶cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).
Immunocytochemistry/ Immunofluorescence - Anti-Bak antibody [AT38E2] (ab104124)

Immunocytochemistry/ Immunofluorescence analysis of BAK in HeLa cells. The cell was stained with ab104124 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot - Anti-Bak antibody [AT38E2] (ab104124)

All lanes : Anti-Bak antibody [AT38E2] (ab104124) at 1/1000 dilution

Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : A549 cell lysate
Lane 5 : Jurkat cell lysate
Lane 6 : MCF7 cell lysate
Lane 7 : PC3 cell lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : goat anti-mouse secondary antibody conjugated to HRP

Predicted band size: 23 kDa
Western blot - Anti-Bak antibody [AT38E2] (ab104124)

Anti-Bak antibody [AT38E2] (ab104124) at 1/500 dilution + Mouse lung lyasate at 35 µg

Secondary
Goat anti-mouse HRP conjugate

Developed using the ECL technique.

Predicted band size: 23 kDa

This image was produced using the ascites version of this antibody.
Immunocytochemistry/ Immunofluorescence - Anti-Bak antibody [AT38E2] (ab104124)

Immunofluorescence staining of Bak in HeLa cells using Hoechst 3342 (Blue) for nucleus staining and ab104124 antibody (1/500) with Texas Red (Red).

This image was produced using the ascites version of this antibody.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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