Astana Biomed Group, an authorized Abcam distributor in Central Asia

HRP Anti-Bak antibody [Y164] (ab199516)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [Y164] to Bak
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-Bak antibody [Y164]
    See all Bak primary antibodies

  • Description

    HRP Rabbit monoclonal [Y164] to Bak

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa whole cell lysate. IHC-P: FFPE human colon (normal) tissue sections.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    Y164

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - HRP Anti-Bak antibody [Y164] (ab199516)
    Western blot - HRP Anti-Bak antibody [Y164] (ab199516)
    All lanes : HRP Anti-Bak antibody [Y164] (ab199516) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : BAK1 knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    ab199516 was shown to specifically react with Bak in wild-type HAP1 cells as signal was lost in BAK1 knockout cells. Wild-type and BAK1 knockout samples were subjected to SDS-PAGE. Ab199516 was incubated overnight at 4°C at 1/5000 dilution. Blots were developed with ECL technique.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Bak antibody [Y164] (ab199516)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Bak antibody [Y164] (ab199516)

    IHC image of Bak staining in a section of formalin-fixed paraffin-embedded normal human colon*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab199516 at 1/125 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

     

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - HRP Anti-Bak antibody [Y164] (ab199516)
    Western blot - HRP Anti-Bak antibody [Y164] (ab199516)
    HRP Anti-Bak antibody [Y164] (ab199516) at 1/5000 dilution + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab199516 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-Bak antibody [Y164] (ab199516)
    HRP Anti-Bak antibody [Y164] (ab199516)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to HRP Anti-Bak antibody [Y164] (ab199516)

© 2021 Astana Biomed Group LLP. All rights reserved.