Anti-Homer1 antibody [EPR15309] (ab184955)
![Anti-Homer1 antibody [EPR15309] (ab184955)](https://www.abcam.com/ps/products/184/ab184955/Images/ab184955-217642-anti-homer1-antibody-epr15309-western-blot.jpg "Anti-Homer1 antibody [EPR15309] (ab184955)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15309] to Homer1
- Suitable for: IP, WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Homer1 antibody [EPR15309]
See all Homer1 primary antibodies -
Description
Rabbit monoclonal [EPR15309] to Homer1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr MouseRatIHC-P HumanIP HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2 and human fetal brain lysates. IP: Human fetal brain lysate. IHC-P: Human gliocytoma tissue. IHC-Fr: Rat and mouse cerebrum tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15309 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)Anti-Homer1 antibody [EPR15309] (ab184955) at 1/5000 dilution + HepG2 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 40 kDa
Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands.
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![Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)](https://www.abcam.com/ps/products/184/ab184955/Images/ab184955-7-anti-homer1-antibody-epr15309-immunohistochemistry-frozen-rat-cerebrum8.jpg)
Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum tissue labeling Homer1 with ab184955 at 1/250 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on rat cerebrum. is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 ug/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] (ab184955)](https://www.abcam.com/ps/products/184/ab184955/Images/ab184955-217640-anti-homer1-antibody-epr15309-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] (ab184955)Immunohistochemical analysis of paraffin-embedded human gliocytoma labeling Homer with ab184955 at 1/500 dilution and HRP polymer for Rabbit IgG. Counterstained with hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-Homer1 antibody [EPR15309] (ab184955)](https://www.abcam.com/ps/products/184/ab184955/Images/ab184955-217638-anti-homer1-antibody-epr15309-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Homer1 antibody [EPR15309] (ab184955)Immunoprecipitation analysis of human fetal brain labeling Homer with ab184955 at 1/50 dilution and Anti-rabbit IgG (HRP) specific to the non reduced form of IgG at 1/1500 dilution (+) or PBS (-)
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![Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)](https://www.abcam.com/ps/products/184/ab184955/Images/ab184955-217641-anti-homer1-antibody-epr15309-western-blot.jpg)
Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)Anti-Homer1 antibody [EPR15309] (ab184955) at 1/20000 dilution + Human fetal brain lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 40 kDa
Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands.
-
![Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)](https://www.abcam.com/ps/products/184/ab184955/Images/ab184955-6-anti-homer1-antibody-epr15309-immunohistochemistry-frozen-mouse-cerebrum8.jpg)
Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling Homer1 with ab184955 at 1/250 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse cerebrum. is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 ug/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Anti-Homer1 antibody [EPR15309] (ab184955)](https://www.abcam.com/ps/products/184/ab184955/Images/ab184955-8-benefits-of-recombinant-antibodies.png)
Anti-Homer1 antibody [EPR15309] (ab184955)
