Preadipocyte Isolation kit (ab196988)
Key features and details
- Sample type: Tissue
Overview
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Product name
Preadipocyte Isolation kit -
Sample type
Tissue -
Species reactivity
Reacts with: Mouse, Rat, Mammals, Other species -
Product overview
Preadipocyte Isolation kit (ab196988) provides the reagents and tools to isolate the stromal vascular fraction from adipose tissue. The resulting stromal vascular fraction is cultured on a tissue culture plate, to which the preadipocytes adhere. The preadipocytes retain the ability to proliferate and differentiate into adipocytes when treated with differentiation-inducing components. The preadipocytes can be used to study the process of adipogenesis, while the differentiated adipocytes can be used to study lipolysis, endocrine activity, cell-signaling and metabolic dysfunction.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 5 g Cell Strainer (100 μm) 10 units Cell Strainer (70 μm) 10 units Collagenase NM, blue 1 x 10ml Collagenase Stop Buffer NM 1 x 90ml PBS 1 x 90ml Red Blood Cell Lysis Buffer 1 x 10ml -
Research areas
Images
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Example data
Preadipocytes from mouse adipose tissue after 4 days of culturing.
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Example dataAdipocytes 7 days after differentiation from preadipocytes using a 3T3-L1 Differentiation Kit. Visible lipid droplets confirm the isolation of preadipocytes.