Anti-BubR1 antibody (ab215351)
Key features and details
- Rabbit polyclonal to BubR1
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-BubR1 antibody
See all BubR1 primary antibodies -
Description
Rabbit polyclonal to BubR1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Macaque monkey, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide within Human BubR1 aa 350-400 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: O60566 -
Positive control
- HeLa whole cell lysate (ab150035); NIH3T3, TCMK-1, CT26.WT and C6 whole cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BubR1 antibody (ab215351)All lanes : Anti-BubR1 antibody (ab215351) at 0.2 µg/ml
Lane 1 : NIH3T3 whole cell lysate
Lane 2 : TCMK-1 whole cell lysate
Lane 3 : 4T1 whole cell lysate
Lane 4 : CT26.WT whole cell lysate
Lane 5 : C6 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 119 kDa
Exposure time: 30 seconds
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Western blot - Anti-BubR1 antibody (ab215351)All lanes : Anti-BubR1 antibody (ab215351) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 119 kDa
Exposure time: 3 seconds
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Immunoprecipitation - Anti-BubR1 antibody (ab215351)Immunoprecipitation analysis of HeLa cell lysate (in RIPA buffer,1.0 mg per IP reaction; 20% of IP loaded) labeling BubR1 using ab215351 at 3 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated Plexin A3, ab191214 was used at 1 μg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
