Anti-HERC2 antibody (ab85832)
Key features and details
- Rabbit polyclonal to HERC2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HERC2 antibody
See all HERC2 primary antibodies -
Description
Rabbit polyclonal to HERC2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 4784-4834 of Human HERC2 (NP_004658.3)
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Positive control
- Whole cell lysate from HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HERC2 antibody (ab85832)All lanes : Anti-HERC2 antibody (ab85832) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 527 kDa
Observed band size: 527 kDa
Additional bands at: 160 kDa, 260 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
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Immunoprecipitation - Anti-HERC2 antibody (ab85832)Immunoprecipitation/ Western Blot
For IP: Lane 1: ab85832 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab85832 at 1µg/ml for WBWhole cell lysate from HeLa cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 30 seconds.
