Aspartate Aminotransferase Activity Assay Kit (Fluorometric) (ab138878)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Other biological fluids, Serum
- Sensitivity: 2 mU/ml
Overview
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Product name
Aspartate Aminotransferase Activity Assay Kit (Fluorometric)
See all Aspartate Aminotransferase kits -
Detection method
Fluorescent -
Sample type
Serum, Other biological fluids -
Assay type
Enzyme activity -
Sensitivity
2 mU/ml -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Aspartate Aminotransferase Activity Assay Kit / AST Assay Kit (Fluorometric) ab138878 provides a quick and sensitive assay for AST activity in biological samples.
In the AST assay protocol, AST catalyzes the reaction of aspartate and α-ketoglutarate to oxaloacetate and glutamate:
Aspartate (Asp) + α-ketoglutarate ? oxaloacetate + glutamate (Glu)
The product L-glutamate is measured by the generation of a red fluorescent product through an enzyme coupled reaction cycle.
The assay signal can be read by a fluorescence microplate reader at Ex/Em = 530-570 nm/590-600 nm (optimal Ex/Em = 540 nm/590 nm). The AST activity assay can detect as little as 2 mU/mL AST in a 100 µL reaction volume. The assay is robust, and can be readily adapted for a wide variety of applications.
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Notes
Aspartate aminotransferase (AST), also called Glutamic Oxaloacetic transaminase (GOT), is a member of transferase family. It catalyzes the reversible transfer of an α-amino group between aspartate and glutamate, and is an important enzyme in amino acid metabolism.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 kit AST Assay Buffer 1 x 10ml AST Assay Enzyme Mixture 1 vial AST Positive Control 1 vial NAD 1 vial -
Research areas
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Function
Plays a key role in amino acid metabolism. -
Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- AATC_HUMAN
- Aspartate aminotransferase
- Aspartate aminotransferase 1
see all
Images
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Sample Standard Curve for Aspartate AminotransferaseAST dose response was measured with ab138878 in a 96-well black plate using a fluorescence microplate reader. As low as 2 mU/ml AST can be detected with 60 min incubation (n=3) at room temperature.
