Aspartate Aminotransferase Activity Assay Kit (ab105135)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 10 mU/well
Overview
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Product name
Aspartate Aminotransferase Activity Assay Kit
See all Aspartate Aminotransferase kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Enzyme activity -
Sensitivity
10 mU/well -
Assay time
1h 00m -
Product overview
Aspartate Aminotransferase Activity Assay Kit (AST assay kit) ab105135 provides a rapid, simple, sensitive and reliable assay for AST activity.
In the AST assay protocol, an amino group is transferred from aspartate to alpha-ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. The glutamate is detected in a reaction that converts a nearly colorless probe to color (λmax = 450 nm).
The AST assay has a detection limit of 10 mU per well.
AST assay protocol summary:
- add samples and standards to wells
- add reaction mix
- incubate at 37ºC and analyze with microplate reader after 10 mins and then again after 60 minsChinese protocol available. See protocols section below.
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Notes
Aspartate aminotransferase (AST), also known as Aspartate transaminase and Glutamate-oxaloacetate transaminase (GOT), is a transaminase (EC 2.6.1.1) similar to the more liver specific alanine transaminase (ALT). Although commonly included clinically as part of a diagnostic liver function test, AST has a broader clinical utility since it may also be elevated in diseases affecting other organs, such as the heart or muscles in myocardial infarction, also in acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease, musculoskeletal diseases and trauma. It catalyzes the reaction: Aspartate + alpha-Ketoglutarate = Oxaloacetate + Glutamate Diagnostically, it is almost always measured in units/liter (U/L).
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Assay Buffer WM 1 x 25ml Developer Red 1 vial Enzyme Mix Green 1 vial Glutamate Standard (0.1M) Yellow 1 x 0.1ml Positive Control Blue 1 vial Substrate Orange 1 vial -
Research areas
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Function
Plays a key role in amino acid metabolism. -
Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- AATC_HUMAN
- Aspartate aminotransferase
- Aspartate aminotransferase 1
see all
Images
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Functional Studies - Aspartate Aminotransferase Activity Assay Kit (ab105135)
AST activity measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1 = 2 min; T2 = 60 min).
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Functional Studies - Aspartate Aminotransferase Activity Assay Kit (ab105135)AST activity measured in human biological fluids showing activity (mU) per mL of tested sample, (T1 = 2 min; T2 = 60 min) with background signals subtracted.
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Functional Studies - Aspartate Aminotransferase Activity Assay Kit (ab105135) Qiu Guihua, et al., Frontiers in Immunology Vol 8. 2017 pg.575, Fig 8, doi:10.3389/fimmu.2017.00575Serum aspartate transaminase (AST) levels were measured using ab105135 and alanine transaminase (ALT) levels were measured using ab105134. Both levels were measured at various time periods post Con A injection. Mean values ± SD are shown (n = 4). ∗P
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Functional Studies - Aspartate Aminotransferase Activity Assay Kit (ab105135)AST activity measured in cell lysates (T1 = 2 min; T2 = 60 min; duplicates (+/- SD), background signal subtracted).
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Functional Studies - Aspartate Aminotransferase Activity Assay Kit (ab105135)AST activity measured in cell culture medium and control medium, background signal subtracted. T1 = 2 min; T2 = 60 min.
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Functional Studies - Aspartate Aminotransferase Activity Assay Kit (ab105135)Standard curve (60 minutes incubation): mean of duplicates (+/- SD) with background reads subtracted.
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Functional Studies - Aspartate Aminotransferase Activity Assay Kit (ab105135)Example of Standard Curve and Positive control detection using ab105135.
