Anti-RBBP4 antibody [EPR3412] (ab92344)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3412] to RBBP4
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-RBBP4 antibody [EPR3412]
See all RBBP4 primary antibodies -
Description
Rabbit monoclonal [EPR3412] to RBBP4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human RBBP4 aa 400 to the C-terminus. The exact sequence is proprietary.
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Positive control
- HeLa, Jurkat, Raji and 293T cell lysates; Human breast carcinoma tissue.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3412 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-RBBP4 antibody [EPR3412] (ab92344) at 1/20000 dilution
Lane 1 : HeLa lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Raji
cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
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ab92344 at 1/250 dilution staining RBBP4 in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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