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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class II

Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (ab248588)

Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (ab248588)
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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7981] to HLA DMB - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-HLA DMB antibody [EPR7981] - BSA and Azide free
    See all HLA DMB primary antibodies
  • Description

    Rabbit monoclonal [EPR7981] to HLA DMB - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Raji, Daudi, Human spleen, 293T with His tagged HLA DMB expression vector transfected cell lysates. Flow Cyt (intra): Raji cells.
  • General notes

    ab248588 is the carrier-free version of ab133640.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 7.40 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7981
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • MHC
    • Class II

Images

  • Western blot - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (ab248588)
    Western blot - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (ab248588)
    All lanes : Anti-HLA DMB antibody [EPR7981] (ab133640) at 1/1000 dilution

    Lane 1 : Raji lysate
    Lane 2 : Daudi lysate
    Lane 3 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 29 kDa



    This data was developed using ab133640, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (ab248588)
    Flow Cytometry - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (ab248588)
    This data was developed using ab133640, the same antibody clone in a different buffer formulation.Flow cytometry analysis of permeabilized Raji cells labelling HLA DMB with ab133640 at 1/100 dilution (red). Rabbit IgG (negative control) shown in green.
  • OI-RD Scanning - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (ab248588)
    OI-RD Scanning - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (ab248588)
    This data was developed using ab133640, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (ab248588)
    Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (ab248588)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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