Anti-Chk1 antibody (ab2845)
Key features and details
- Goat polyclonal to Chk1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Chk1 antibody
See all Chk1 primary antibodies -
Description
Goat polyclonal to Chk1 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phospho peptide (Human), which represents a portion of human Chk 1 encoded within exon 8.
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Positive control
- HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Chk1 antibody (ab2845)ab2845 at a 1:10000 dilution staining ~57 kDa Chk 1 in HeLa whole cell lysate (30
µ g / lane, +/- 10 Gy IR, +/- calf intestinal phosphatase treatment (CIP)) by Western blot (ECL, 30 seconds exposure). ab2845 at a 1:10000 dilution staining ~57 kDa Chk 1 in HeLa whole cell lysate (30µg / lane, +/- 10 Gy IR, +/- calf intestinal phosphatase treatment (CIP)) by Western blot (ECL, 30 seconds exposure). -
Western blot - Anti-Chk1 antibody (ab2845) This image is courtesy of an anonymous AbreviewAll lanes : Anti-Chk1 antibody (ab2845) at 1/10000 dilution
Lane 1 : Whole cell lysate of HT29 tumour cell line, untreated
Lane 2 : Whole cell lysate of HT29 tumour cell line, treated with DMSO
Lane 3 : Whole cell lysate of HT29 tumour cell line, treated with 50nM SN38 for 6h
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP conjugated cow anti-goat antibody at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
