Anti-Chk1 (phospho S301) antibody (ab61023)
Key features and details
- Rabbit polyclonal to Chk1 (phospho S301)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Chk1 (phospho S301) antibody
See all Chk1 primary antibodies -
Description
Rabbit polyclonal to Chk1 (phospho S301) -
Host species
Rabbit -
Specificity
ab61023 detects endogenous levels of Chk1 only when phosphorylated at serine 301. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic phosphopeptide derived from human Chk1 around the phosphorylation site of serine 301 (D-F-SP-P-V).
-
Positive control
- Extracts from 293 cells
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Chk1 (phospho S301) antibody (ab61023)All lanes : Anti-Chk1 (phospho S301) antibody (ab61023) at 1/500 dilution
Lane 1 : Extracts from 293 cells
Lane 2 : Extracts from 293 cells with Immunizing peptide
Predicted band size: 54 kDa
Observed band size: ~100,50 kDa why is the actual band size different from the predicted?
