Anti-CD15 antibody [SP159] (ab135377)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP159] to CD15
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Species independent
Overview
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Product name
Anti-CD15 antibody [SP159]
See all CD15 primary antibodies -
Description
Rabbit monoclonal [SP159] to CD15 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species -
Immunogen
Other Immunogen Type corresponding to Human CD15. CD15 purified from U937 cells.
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Positive control
- IHC-P: Human Hodgkins Lymphoma, spleen, cervix carcinoma, and Mouse kidney tissuesFlow Cyt: THP-1 cells
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General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP159 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling CD15 with ab135377 at 1/50 dilution (3.14 µg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on proximal tubular epithelial cells in the mouse kidney, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab135377 for 30 mins at room temperature. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervix carcinoma tissue sections labeling CD15 with ab135377 at 1/50 dilution (3.14 µg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human cervix carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab135377 for 30 mins at room temperature. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Hodgkin's lymphoma tissue sections labeling CD15 with ab135377 at 1/50 dilution (3.14 µg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human Hodgkin's lymphoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab135377 for 30 mins at room temperature. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD15 with ab135377 at 1/50 dilution (3.14 µg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab135377 for 30 mins at room temperature. -
Flow cytometry analysis of THP-1 (human acute monocytic leukemia) labeling CD15 with purified ab135377 at 1/20 dilution (7.85 µg/ml) (red). Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Hodgkin’s Lymphoma tissue labelling CD15 with ab135377 at 1/100 dilution.
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Flow cytometric analysis of rabbit anti-CD15 (SP159) antibody ab135377 (1/200) in HL-60 cells(green) compared to negative control of rabbit IgG (blue).
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