Anti-Chk1 antibody (ab17191)
Key features and details
- Rabbit polyclonal to Chk1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Chk1 antibody
See all Chk1 primary antibodies -
Description
Rabbit polyclonal to Chk1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIP HumanWB Human
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Immunogen
Synthetic peptide (Human). Epitope is in the region between 250 and 300 of Chk1 (Swiss-prot: O14757).
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Positive control
- IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Chk1 antibody (ab17191)Detection of Chk1 by immunoprecipitation of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Lane 1: Anti-Chk1 antibody (ab17191) at 6 µg/mg lysate, Lane 2: Anti-Chk1 antibody (ab 17191) at 6 µg/mg lysate, Lane 3: Control IgG was used as a negative control. Detection: Chemiluminescence with an exposure time of 1 seconds.
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Immunocytochemistry/ Immunofluorescence - Anti-Chk1 antibody (ab17191)Immunocytochemistry/Immunofluorescence analysis of NBF-fixed HeLa cells treated with neocarzinostatin labelling Chk1 with ab17191 at 1/1000 (1µg/ml) . A FITC-conjugated anti-rabbit IgG (1/100) was used as the secondary antibody. -
Western blot - Anti-Chk1 antibody (ab17191)Samples: whole cell lysate (20 micrograms (mcg)) from Hela cells.
ab17191 was used at the concentrations indicated on the image.
Samples: whole cell lysate (20 micrograms (mcg)) from Hela cells.
ab17191 was used at the concentrations indicated on the image.
