Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944)
![Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944)](https://www.abcam.com/ps/products/201/ab201944/Images/ab201944-243118-anti-phospholipase-a2-xii-antibody-3h2c11-western-blot.jpg "Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944)")
Key features and details
- Mouse monoclonal [3H2C11] to Phospholipase A2 XII/PLA2G12A
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11]
See all Phospholipase A2 XII/PLA2G12A primary antibodies -
Description
Mouse monoclonal [3H2C11] to Phospholipase A2 XII/PLA2G12A -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human Phospholipase A2 XII/PLA2G12A aa 1 to the C-terminus. (Purified from E.coli).
Database link: Q9BZM1 -
Positive control
- Human Phospholipase A2 XII/PLA2G12A (aa21-189) recombinant protein; Phospholipase A2 XII/PLA2G12A (aa21-189)-hIgGFc transfected HEK293 cell lysate; HeLa cells; Human liver cancer tissue.
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General notes
This product was previously labelled as Phospholipase A2 XII
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3H2C11 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944)Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944) at 1/500 dilution + Human Phospholipase A2 XII/PLA2G12A (aa21-189) recombinant protein
Predicted band size: 21 kDaExpected MWt is 44.5 kDa.
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![Western blot - Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944)](https://www.abcam.com/ps/products/201/ab201944/Images/ab201944-243117-anti-phospholipase-a2-xii-antibody-3h2c11-western-blot.jpg)
Western blot - Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944)All lanes : Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Phospholipase A2 XII/PLA2G12A (aa21-189)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 21 kDa
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![Flow Cytometry - Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944)](https://www.abcam.com/ps/products/201/ab201944/Images/ab201944-243116-anti-phospholipase-a2-xii-antibody-3h2c11-flow-cytometry.jpg)
Flow Cytometry - Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944)Flow cytometric analysis of HeLa cells labeling Phospholipase A2 XII/PLA2G12A with ab201944 at 1/200 dilution (green) compared to a negative control (red).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944)](https://www.abcam.com/ps/products/201/ab201944/Images/ab201944-243114-anti-phospholipase-a2-xii-antibody-3h2c11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944)Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling Phospholipase A2 XII/PLA2G12A with ab201944 at 1/200 dilution, with DAB staining.
