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Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944)

Key features and details

  • Mouse monoclonal [3H2C11] to Phospholipase A2 XII/PLA2G12A
  • Suitable for: Flow Cyt, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11]
    See all Phospholipase A2 XII/PLA2G12A primary antibodies

  • Description

    Mouse monoclonal [3H2C11] to Phospholipase A2 XII/PLA2G12A

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human Phospholipase A2 XII/PLA2G12A aa 1 to the C-terminus. (Purified from E.coli).
    Database link: Q9BZM1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human Phospholipase A2 XII/PLA2G12A (aa21-189) recombinant protein; Phospholipase A2 XII/PLA2G12A (aa21-189)-hIgGFc transfected HEK293 cell lysate; HeLa cells; Human liver cancer tissue.

  • General notes

     This product was previously labelled as Phospholipase A2 XII

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944)
    Western blot - Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944)
    Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944) at 1/500 dilution + Human Phospholipase A2 XII/PLA2G12A (aa21-189) recombinant protein

    Predicted band size: 21 kDa



    Expected MWt is 44.5 kDa.

  • Western blot - Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944)
    Western blot - Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944)
    All lanes : Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Phospholipase A2 XII/PLA2G12A (aa21-189)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 21 kDa

  • Flow Cytometry - Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944)
    Flow Cytometry - Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944)

    Flow cytometric analysis of HeLa cells labeling Phospholipase A2 XII/PLA2G12A with ab201944 at 1/200 dilution (green) compared to a negative control (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase A2 XII/PLA2G12A antibody [3H2C11] (ab201944)

    Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling Phospholipase A2 XII/PLA2G12A with ab201944 at 1/200 dilution, with DAB staining.

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