Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ALKBH1 antibody [EPR6175(2)] (ab128895)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6175(2)] to ALKBH1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-ALKBH1 antibody [EPR6175(2)]
    See all ALKBH1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6175(2)] to ALKBH1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ALKBH1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • A431, Jurkat, A549, K562, Raw 264.7, and NIH3T3 cell lysates, Human breast carcinoma tissue

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 6.10 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR6175(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ALKBH1 antibody [EPR6175(2)] (ab128895)
    Western blot - Anti-ALKBH1 antibody [EPR6175(2)] (ab128895)
    All lanes : Anti-ALKBH1 antibody [EPR6175(2)] (ab128895) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : ALKBH1 knockout HAP1 whole cell lysate
    Lane 3 : K562 whole cell lysate
    Lane 4 : A549 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 43 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab128895 observed at 43 kDa. Red - loading control, ab7291, observed at 50 kDa.

    ab128895 was shown to specifically react with in wild-type HAP1 cells as signal was lost in ALKBH1 knockout cells. Wild-type and ALKBH1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab128895 and ab7291 (Mouse anti Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ALKBH1 antibody [EPR6175(2)] (ab128895)
    Western blot - Anti-ALKBH1 antibody [EPR6175(2)] (ab128895)
    All lanes : Anti-ALKBH1 antibody [EPR6175(2)] (ab128895) at 1/1000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : Raw 264.7 cell lysate
    Lane 6 : NIH3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALKBH1 antibody [EPR6175(2)] (ab128895)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALKBH1 antibody [EPR6175(2)] (ab128895)

    ab128895, at a dilution of 1/50, staining ALKBH1 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-ALKBH1 antibody [EPR6175(2)] (ab128895)
    OI-RD Scanning - Anti-ALKBH1 antibody [EPR6175(2)] (ab128895)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-ALKBH1 antibody [EPR6175(2)] (ab128895)
    Anti-ALKBH1 antibody [EPR6175(2)] (ab128895)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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